Azidoacetic acid NHS ester Chemical Properties

Melting point:

132-133o C

storage temp. 

-20°C

solubility 

Chloroform (Slightly)

form 

powder or crystals

color 

White to Off-White

InChI

InChI=1S/C6H6N4O4/c7-9-8-3-6(13)14-10-4(11)1-2-5(10)12/h1-3H2

InChIKey

FENNDBOWHRZLTQ-UHFFFAOYSA-N

SMILES

O(N1C(CCC1=O)=O)C(=O)CN=[N+]=[N-]

Azidoacetic acid NHS ester Usage And Synthesis

Description

Azidoacetic acid NHS ester is a compound containing an azide group and an NHS ester. The azide group can react with alkyne, BCN, DBCO via Click Chemistry to yield a stable triazole linkage. The NHS ester can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

Uses

Azidoacetic acid NHS ester is a simple azido-containing reagent with an N-hydroxysuccinimide (NHS) ester that can be reacted with primary amines at pH 7-9 forming a stable amide bond. The azide allows for chemoselective ligation with an alkyne or cyclooctyne. It is generally used to functionalize amines and amino acids with azidoacetyl groups. It is generally used in click chemistry applications.

reaction suitability

reagent type: cross-linking reagent

IC 50

Alkyl/ether

Azidoacetic acid NHS ester(824426-32-6)Related Product Information

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