Chapso Chemical Properties

Melting point:

184-186 °C(lit.)

Density 

1.0453 (rough estimate)

refractive index 

1.6900 (estimate)

storage temp. 

-20°C

solubility 

H₂O: 10 mg/mL at 20 °C, clear, colorless

form 

White solid

color 

White crystalline

Odor

Odorless

Water Solubility 

10 g/l at 20 °C

Merck 

13,2052

BRN 

5842642

CAS DataBase Reference

82473-24-3(CAS DataBase Reference)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36

WGK Germany 

3

F 

3-10

HS Code 

2924297099

MSDS

• Language:English Provider:SigmaAldrich

Chapso Usage And Synthesis

Chemical Properties

Chapso is a white crystalline powder. It is a zwitterionic detergent similar to Chaps, however, Chapso is more soluble and particularly better for the solubilization of integral membrane proteins.

Uses

Chapso is a nondenaturing biological detergent with characteristics similar to CHAPS, although it is more soluble due to a more polar head group. Useful for the solubilization of integral membrane proteins.

Application

3-(cholamidopropyl)(dimethylammonio)-2-hydroxy-1-propanesulfonate (CHAPSO) may be used in bicelle preparation for β2-adrenergic receptor reconstitution.
CHAPSO has been used:
in a study to assess the processes of liposome solubilization and reconstitution of two transport proteins
in a study to investigate a novel bicelle system for orienting macromolecules over a wide temperature range
as a mixture with dilauroyl phosphatidylcholine (DLPC) in solid-state nuclear magnetic resonance (NMR) technique to study membrane-associated proteins
A nondenaturing zwitterionic detergent with characteristics similar to CHAPS, although it is more soluble due to a more polar head group. Useful for the solubilization of integral membrane proteins.

General Description

CHAPSO is a non-denaturing zwitterionic bile salt derivative, derived from Chaps by the addition of a functional hydroxyl group.

Purification Methods

Dissolve this zwitterionic detergent (~ 60g) in 40% aqueous MeOH and stir it with the mixed-bed ion-exchange resin RG-501 x 8 (100g, Bio-Rad) until the pH of the supernatant is ~ 7. Filter off the resin and evaporate the filtrate to dryness under reduced pressure. Check for homogeneity by TLC on silica gel G in 95% MeOH/5% NH4OH and visualize the spot with iodine vapour (see CHAPS above). [Hjelmeland et al. Anal Biochem 130 72 1983, Matuo et al. Methods Enzymol 198 155 1991.]

Chapso(82473-24-3)Related Product Information

• N,N-Dimethyl-1,4-phenylenediamine
• 4-Amino-benzenesulfonic acid monosodium salt
• Sulfanilic acid
• Sulfamic acid monosodium salt
• Nickel bis(sulphamidate)
• Sodium allylsulfonate
• Sulfamic acid
• CHAPS
• CHLOROPHOSPHONAZO III
• Cocamidopropyl hydroxysultaine
• N-[3-(DIMETHYLAMINO)PROPYL]STEARAMIDE
• Cholesterol
• LAURYLAMINO PROPYLAMINE
• 3-(Cholamidopropyl)-1,1-dimethamine
• NDSB-195
• Chapso
• N-(3-COCOAMIDOPROPYL)-N,N-DIMETHYL-N-(3-SULFOPROPYL)AMMONIUM BETAINE
• N-(3-aminopropyl)stearamide