Azide-PEG3-Desthiobiotin Chemical Properties

storage temp. 

Storage temp. 2-8°C

form 

clear liquid

color 

Colorless to Yellow to Orange

Safety Information

HS Code 

2933.29.9000

Azide-PEG3-Desthiobiotin Usage And Synthesis

Description

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd?= 1011?vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

Uses

Azide-PEG3-Desthiobiotin is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1]. Azide-PEG3-Desthiobiotin is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

IC 50

PEGs

References

[1] An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562 DOI:10.1016/j.ebiom.2018.09.005

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