Azide-PEG3-Desthiobiotin Chemical Properties

form 

clear liquid

color 

Colorless to Yellow to Orange

Safety Information

HS Code 

2933.29.9000

Azide-PEG3-Desthiobiotin Usage And Synthesis

Description

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd?= 1011?vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

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