BIOTIN-NHS Chemical Properties

Melting point:

212-214 °C

Density 

1.45±0.1 g/cm3(Predicted)

storage temp. 

-20°C

solubility 

DMF: ≤50 mg/mL Stable for at least one month in dry DMF

form 

powder

pka

13.89±0.40(Predicted)

color 

White to off-white

BRN 

4720781

Stability:

Stable. Incompatible with strong oxidizing agents.

CAS DataBase Reference

35013-72-0(CAS DataBase Reference)

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

F 

10-23

HS Code 

2934 99 90

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

BIOTIN-NHS Usage And Synthesis

Description

N-Hydroxysuccinimide (NHS) activates carboxylic acid groups (on biotin) to facilitate coupling reactions. Biotin-NHS is a compound used to attach biotin to primary amines under alkaline conditions (pH~8-9). For example, lysines on the surface of proteins, including antibodies, are ideal targets for biotinylation with this compound.

Chemical Properties

White Solid

Uses

amine reactive biotinylation reagent

Uses

Biotin-NHS is used in the biotinylation of proteins and peptides. Couples with primary amine in the pH range 6.5-8.5.

Uses

Amino reactive biotin reagent for preparing biotinylated surfaces or polypeptides.

Purification Methods

Recrystallise the ester from refluxing isoPrOH and dry it in a vacuum over P2O5 + KOH. [Jasiewicz et al. Exp Cell Biol 100 213 1976.]

BIOTIN-NHS(35013-72-0)Related Product Information

• Sulfo-NHS-Biotin
• BIOTIN-XX-NHS
• D-BIOTIN P-NITROPHENYL ESTER
• Succinimidyl 6-(biotinamido)hexanoate
• N-Hydroxysuccinimide
• methyl 5-[(1S,2S,5R)-7-oxo-3-thia-6,8-diazabicyclo[3.3.0]oct-2-yl]pent anoate
• BIOTIN HYDRAZIDE
• (+)-BIOTIN-EPSILON-AMINOCAPROIC ACID
• BIOTIN DISULFIDE N-HYDROXY-SUCCINIMIDE ESTER
• Biotin-NHCH2CH2-SS-Pyr
• Biotin-SLC-NHS
• Succinimide
• BIOTIN-NHS
• DESTHIOBIOTIN N-HYDROXYSUCCINIMIDYL ESTER
• 2,5-Dioxopyrrolidin-1-yl octanoate
• D-DESTHIOBIOTIN
• D-biotin-d-sulfoxide
• 7,8-DIAMINOPELARGONIC ACID