Basic information description Uses Health Hazards Safety Supplier Related
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Ethidium bromide

Basic information description Uses Health Hazards Safety Supplier Related

Ethidium bromide Basic information

Product Name:
Ethidium bromide
Synonyms:
  • 3,8-diaMino-5-ethyl-6-phenylphenanthridin-5-iuM broMide
  • EthidiuM broMide dropper bottle,5Ml
  • 2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide, 1% aqueous solution
  • Ethidinium bromide, 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide
  • EthidiuM BroMide, OMniPur(R)
  • EthidiuM broMide, pure, 95% 1GR
  • EthidiuM broMide, pure, 95% 5GR
  • Dromilac
CAS:
1239-45-8
MF:
C21H20BrN3
MW:
394.31
EINECS:
214-984-6
Product Categories:
  • marker
  • Amines
  • Aromatics
  • Intermediates & Fine Chemicals
  • Pharmaceuticals
  • Phenanthridine
  • Purines
Mol File:
1239-45-8.mol
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Ethidium bromide Chemical Properties

Melting point:
260-262 °C (dec.)(lit.)
Density 
1.3739 (rough estimate)
refractive index 
1.6700 (estimate)
Flash point:
>100°C
storage temp. 
2-8°C
solubility 
H2O: 10 mg/mL, opaque, strongly red
form 
powder
color 
Red to dark purple
Odor
Odorless solid
Water Solubility 
40 g/L (25 ºC)
λmax
518 nm, 210 nm, 285 nm, 316 nm, 343 nm, 480 nm, 525 nm
Merck 
14,4731
BRN 
3642536
Stability:
Stable. Incompatible with strong oxidizing agents.
Biological Applications
Nucleic acid hybridization; detecting nucleic acids,cells,cancer cells,human cytomegalovirus,hydrogenase A (hydA) of Clostridia,influenza A virus,oligonucleotides,viable Plesiomonas shigelloides; apoptosis assay; nucleic acid quantification
Major Application
Electroluminescent displays;photoresists
CAS DataBase Reference
1239-45-8(CAS DataBase Reference)
EPA Substance Registry System
Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1239-45-8)
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Safety Information

Hazard Codes 
T,T+
Risk Statements 
23-68-36/37/38-26-21/22-22
Safety Statements 
36/37-45-36/37/39-28A-26-22-28-63
RIDADR 
UN 2811 6.1/PG 1
WGK Germany 
3
RTECS 
SF7950000
8-9
HazardClass 
6.1
PackingGroup 
I
HS Code 
29339990
Hazardous Substances Data
1239-45-8(Hazardous Substances Data)
Toxicity
Intercalating dye widely used to stain DNA in gels and gradients. The DNA can be visualized readily by irradiation with ultraviolet light, as little as 0.5 μg of DNA being detectable by such methods. Ethidium bromide is also added to cesium chloride density gradients, since as an intercalating molecule it binds more readily to linear DNA than to closed collinear circles of DNA (such as plasmids). Binding of ethidium bromide reduces the density of DNA, thus covalent circles of DNA have higher densities at saturating concentrations of ethidium bromide, permitting the separation of plasmid DNA. Ethidium bromide is a putative carcinogen.

MSDS

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Ethidium bromide Usage And Synthesis

description

Ethidium Bromide (EtBr), commonly used in research laboratories as a stain for the visualization of nucleic acids in electrophoresis gels, is a toxic chemical and a potent mutagen. When used in nucleic acid staining, ethidium bromide fluoresces a red-orange to pink color under ultraviolet light and with increased fluorescence when bound to double-stranded DNA. While it is not specifically regulated as a hazardous waste, the mutagenic properties may present health hazards and disposal concerns if it is not managed properly in the laboratory.

Uses

Ethidium bromide (EtBr) is a fluorescent dye widely used in molecular biology research. Early usage was as a veterinary trypanocide. It is a mutagenic compound that intercalates double-stranded DNA and RNA. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA, 25-fold on binding double-stranded DNA (although histones block binding of EtBr to DNA). Ethidium bromide has been used in multiple fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of the binding to DNA, EtBr is a powerful inhibitor of DNA polymerase.
Molecular Biology-grade Powder is suitable for use in gel electrophoresis and DNA isolation procedures.
Aqueous Solution (10 mg/mL) is suitable for use in gel electrophoresis and DNA isolation procedures.
Molecular Biology-grade Aqueous Solution (500 mg/mL) is suitable for use in gel electrophoresis.

Health Hazards

EtBr is a potent mutagen (may cause genetic damage), and moderately toxic after an acute exposure. EtBr can be absorbed through skin, so it is important to avoid any direct contact with the chemical. EtBr is an irritant to the skin, eyes, mouth, and upper respiratory tract. It should be stored away from strong oxidizing agents in a cool, dry place, and the container must be kept undamaged and tightly closed.

Chemical Properties

Purple/maroon crystalline powder

Uses

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprotozoal (Trypanosoma).

Uses

Intercalating agent and fluorescent label for DNA.

Uses

antiprotozoal, intercalcates with DNA

Health Hazard

Acute toxic effects from exposure to ethidium bromide have not been thoroughly investigated. Ethidium bromide is irritating to the eyes, skin, mucous membranes, and upper respiratory tract. Although there is no evidence for the carcinogenicity or teratogenicity of this substance in humans, ethidium bromide is strongly mutagenic and therefore should be regarded as a possible carcinogen and reproductive toxin

Flammability and Explosibility

Ethidium bromide does not pose a flammability hazard (NFPA rating = 1).

Biochem/physiol Actions

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Safety Profile

Because ethidium bromide can bind with DNA, it is highly toxic as a mutagen. It may potentially cause carcinogenic or teratogenic effects, although no scientific evidence showing either health effect has been found. Exposure routes of ethidium bromide are inhalation, ingestion, and skin absorption. An acute exposure to ethidium bromide causes irritation of the mouth, upper respiratory tract, skin, and eyes.

storage

Because of its mutagenicity, stock solutions of this compound should be prepared in a fume hood, and protective gloves should be worn at all times while handling this substance. Operations capable of generating ethidium bromide dust or aerosols of ethidium bromide solutions should be conducted in a fume hood to prevent exposure by inhalation.

Purification Methods

Crystallise it from MeOH or EtOH [Lamos et al. J Am Chem Soc 108 4278 1986]. Its solubility in H2O is 1%. [Beilstein 22/11 V 352.] POSSIBLE CARCINOGEN.

Incompatibilities

No incompatibilities are known.

Waste Disposal

Excess ethidium bromide and waste material containing this substance should be placed in an appropriate container, clearly labeled, and handled according to your institution's waste disposal guidelines.

Ethidium bromideSupplier

Hunan Hui Bai Shi Biotechnology Co., Ltd. Gold
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0731-85526065 19974514128
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Products Intro
Product Name:EB
CAS:1239-45-8
Purity:99% HPLC Package:25kg;5KG;1KG
Jiangsu Boquan Biotechnology Co., Ltd.
Tel
13276599337
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1983403558@qq.com
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Product Name:Ethidium bromide, 95%
CAS:1239-45-8
Purity:95% Package:100g/RMB 3500
J & K SCIENTIFIC LTD.
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010-82848833 400-666-7788
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jkinfo@jkchemical.com
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Product Name:Ethidium bromide, 95%
CAS:1239-45-8
Purity:95% Package:1G;5G
Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
18621169109
Email
market03@meryer.com
Products Intro
Product Name:EthidiuM BroMide
CAS:1239-45-8
Purity:>90.0%(T) Remarks:E0370
Alfa Aesar
Tel
400-6106006
Email
saleschina@alfa-asia.com
Products Intro
Product Name:EthidiuM broMide, 98% (dry wt.)
CAS:1239-45-8
Package:1g Remarks:L07482