N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride Chemical Properties

Melting point:

222-224 °C(lit.)

Boiling point:

378.54°C (rough estimate)

Density 

1.1961 (rough estimate)

refractive index 

1.6400 (estimate)

storage temp. 

room temp

solubility 

H2O: soluble500mg/10 mL

form 

powder

color 

off-white to gray

Water Solubility 

SOLUBLE

Sensitive 

Light Sensitive

BRN 

5132096

Stability:

Stable. Incompatible with strong oxidizing agents, strong bases.

CAS DataBase Reference

637-01-4(CAS DataBase Reference)

EPA Substance Registry System

1,4-Benzenediamine, N,N,N',N'-tetramethyl-, dihydrochloride (637-01-4)

Safety Information

Hazard Codes 

Xi,Xn

Risk Statements 

36/37/38-20/21/22-40

Safety Statements 

26-36-36/37-22-36/37/39

RIDADR 

2811

WGK Germany 

3

F 

8

TSCA 

Yes

HazardClass 

6.1(a)

PackingGroup 

II

HS Code 

29215190

MSDS

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N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride Usage And Synthesis

Chemical Properties

Off white to gray powder

Uses

N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride (TMPD) is an oxidizable compound that serves as a reducing co-substrate for heme peroxidases. It is also used as a test reagent in microbiology for the classification of cytochrome oxidase positive aerobic microorganisms. It finds application as a detection of peroxidases on polyacrylamide gels.

Uses

N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride has been used to determine the oxidase activity displayed by various microorganisms.

General Description

N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride (TMPD) is a redox mediator used in the oxidase test for detecting bacterial cytochrome c oxidases. TMPD is commonly used to perform the oxidase test for the colorimetric identification of pathogenic and non-pathogenic bacteria.

Purification Methods

Crystallise the salt from isopropyl or n-butyl alcohols, saturated with HCl. Treat it with aqueous NaOH to give the free base (see previous entry) which is filtered, dried and sublimed in a vacuum. [Guarr et al. J Am Chem Soc 107 5104 1985, Beilstein 13 H 74.]

N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride(637-01-4)Related Product Information

• N,N-Dimethyl-1,4-phenylenediamine
• Cysteamine hydrochloride
• 1-Methylpyrrolidine
• o-Phenylenediamine
• LITHIUM PERIODATE
• Benzhydrylamine
• 2-Phenylethylamine hydrochloride
• Burgess reagent
• p-Phenylenediamine
• BLOCKING REAGENT GE
• N-Methyl-2-pyrrolidone
• Methoxyammonium chloride
• 2,3,5,6-TETRAMETHYL-1,4-PHENYLENEDIAMINE
• 1-Methylimidazole
• 2-Aminoethylammonium chloride
• p-Phenylenediamine dihydrochloride
• Dihydrochloride
• Hexamethylenetetramine