4-NITROPHENYL-N-ACETYL-BETA-D-GLUCOSAMINIDE Chemical Properties

Melting point:

210-212 °C

Boiling point:

477.77°C (rough estimate)

alpha 

-14～-18°(D/20℃) (c=0,H2O)

Density 

1.3038 (rough estimate)

refractive index 

1.5110 (estimate)

storage temp. 

-20°C

solubility 

H₂O: 5 mg/mL, clear, colorless to very faintly yellow

pka

12.76±0.70(Predicted)

form 

Fine Powder

color 

White to off-white

Water Solubility 

water: 5mg/mL, clear, colorless to very faintly yellow

λmax

400nm(Phosphate buffer sol.)(lit.)

BRN 

96193

CAS DataBase Reference

3459-18-5(CAS DataBase Reference)

Safety Information

Hazard Codes 

Xi

Safety Statements 

22-24/25

WGK Germany 

3

F 

10-21

HS Code 

29329990

MSDS

• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich

4-NITROPHENYL-N-ACETYL-BETA-D-GLUCOSAMINIDE Usage And Synthesis

Chemical Properties

Slightly Off-White Crystalline Solid

Uses

substrate for a-L-fucosidases

Uses

Useful substrate for the rapid colorimetric assay of N-Acetyl-beta-glucosaminidase in human urine.

Uses

4-Nitrophenyl-N-acetyl-β- D-glucosaminide is a useful substrate for the rapid colorimetric assay of N-Acetyl-beta-glucosaminidase in human urine

Definition

ChEBI: An N-acetyl-beta-D-glucosaminide in which the anomeric hydroxy hydrogen is replaced by a 4-nitrophenyl group.

Biological Activity

4-Nitrophenyl N-acetyl-β-D-glucosaminide (NP-GlcNAc) is a widely used hexosaminidase substrate. Enzymatic action cleaves the glycosidic bond and forms 4-nitrophenolate (pNP) which is measured at 405 nm. NP-GlcNAc can also be used in combination with diethylaminoethyl-a-cyclodextrin (DEn-CD) for a rapid and accurate rate assay method for b-N-acetylglucosaminidase. The cyclodextrin derivative is used as an additive to ionize 4-nitrophenol to yellow-colored 4-nitrophenoxide at a pH near 5, the pH optimum for the enzyme.

4-NITROPHENYL-N-ACETYL-BETA-D-GLUCOSAMINIDE(3459-18-5)Related Product Information

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• 4-NITROPHENYL 2-ACETAMIDO-2-DEOXY-(4-O-A-L-FUCOPYRANOSYL)-BETA-D-GLUCOPYRANOSIDE
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