CD4 ANTIBODY Usage And Synthesis

Applications

Blocking and staining antibody. Blocks blinding of HIV to CD4. For staining, use 10 µl/1,000,000 cells. Titer for blocking T cell activation should be determined by the investigator.

Immunogen

Purified human PBL CD4+ T cells.

Background

CD4 is a cell-surface glycoprotein found on the mature helper T cells and immature thymocytes, as well as on monocytes and macrophages. (Some cytotoxic T cells have CD4 protein as well.) Normally, about 65% of T cells in the blood are CD4+ (have CD4 protein protruding from their membrane). A mature T cell with either have CD4 or CD8, but not both. During one stage of development T cells develop CD4 and CD8 receptors, but they eventually are differentiated in the thymus and become more specialized.

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