CI 51180 Chemical Properties

Melting point:

>300 °C (dec.)(lit.)

storage temp. 

room temp

solubility 

Soluble in water, ethanol

Colour Index 

51180

form 

Powder

color 

Dark green to black

PH Range

10.1(BLUE)-11.1(RED)

Water Solubility 

Soluble in ethanol, and water (50 g/L) at 25°C.

λmax

633 nm

Biological Applications

Detecting microorganisms; treating virus infectious diseases; photodynamic therapy

InChI

InChI=1S/C20H20N3O.H2O4S/c1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h5-12H,3-4,21H2,1-2H3;(H2,1,2,3,4)/q+1;/p-2

InChIKey

MMYKGGIXVBGGSZ-UHFFFAOYSA-L

SMILES

NC1C=C2[O+]=C3C=C(N(CC)CC)C=CC3=NC2=C2C=CC=CC=12.S([O-])([O-])(=O)=O

Safety Information

Hazard Codes 

Xi

Safety Statements 

22-24/25

WGK Germany 

3

RTECS 

DJ1931000

HS Code 

3212900000

Toxicity

mouse,LD,intraperitoneal,> 500mg/kg (500mg/kg),"Summary Tables of Biological Tests," National Research Council Chemical-Biological Coordination Center. Vol. 4, Pg. 46, 1952.

MSDS

• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

CI 51180 Usage And Synthesis

Chemical Properties

dark green to black powder

Uses

Nile Blue A is used in the Lillie and Fullmer method for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue. Nile blue A is also useful for staining fats and phospholipids. Dyes and metabolites.

Uses

Nile blue A is suitable for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue, and in a histochemical method for lipids in frozen sections.

Uses

Nile Blue A is a stain used for discriminating between melanins and lipofuscins in paraffin sections of animal tissue

General Description

Nile Blue A is aminodiethylaminonaphthophenoxazonium sulfate employed in biology and histology. It is a highly fluorescent, and photostable organic dye used to dye clothing, and is for use in lipid histochemistry.

Biochem/physiol Actions

Nile blue A stains acidic components, such as phospholipids, nucleic and fatty acids by imparting a dark blue color, while neutral lipids, namely triglycerides, are stained pink, or red.

Purification Methods

Crystallise the dye from aqueous AcOH. It has UV: at 630nm (96% aqueous max EtOH) and 635nm (H2O). The betaine has UV: at 513nm (EtOH). [Crossley et al. J Am Chem Soc 74 max 57, 578 1952, Merill & Spencer J Am Chem Soc 70 3683 1948, Beilstein 27 II 457, 27 III/IV 5166.]

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