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CI 51180

Basic information Safety Supplier Related

CI 51180 Basic information

Product Name:
CI 51180
Synonyms:
  • 5-amino-9-(diethylamino)-benzo(a)phenoxazin-7-iusulfate(2:1)
  • 9-(diethylamino)-5-imino-5h-benzo(a)phenazoxazinsulfate(2:1)
  • Benzo[.alpha.]phenoxazin-7-ium,5-amino-9-(diethylamino)-,sulfate(2:1)
  • nileblueasulphate
  • BASIC BLUE 12
  • BASIC BLUE 12 SULFATE SALT
  • CI NO 51180
  • CI 51180
CAS:
3625-57-8
MF:
C20H20N3O5S-
MW:
414.46
EINECS:
222-832-5
Product Categories:
  • Phenoxazine
  • marker
Mol File:
3625-57-8.mol
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CI 51180 Chemical Properties

Melting point:
>300 °C (dec.)(lit.)
storage temp. 
room temp
solubility 
Soluble in water, ethanol
Colour Index 
51180
form 
Powder
color 
Dark green to black
PH Range
10.1(BLUE)-11.1(RED)
Water Solubility 
Soluble in ethanol, and water (50 g/L) at 25°C.
λmax
633 nm
Biological Applications
Detecting microorganisms; treating virus infectious diseases; photodynamic therapy
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Safety Information

Hazard Codes 
Xi
Safety Statements 
22-24/25
WGK Germany 
3
RTECS 
DJ1931000
HS Code 
3212900000
Toxicity
mouse,LD,intraperitoneal,> 500mg/kg (500mg/kg),"Summary Tables of Biological Tests," National Research Council Chemical-Biological Coordination Center. Vol. 4, Pg. 46, 1952.

MSDS

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CI 51180 Usage And Synthesis

Chemical Properties

dark green to black powder

Uses

Nile Blue A is used in the Lillie and Fullmer method for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue. Nile blue A is also useful for staining fats and phospholipids. Dyes and metabolites.

Uses

Nile blue A is suitable for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue, and in a histochemical method for lipids in frozen sections.

Uses

Nile Blue A is a stain used for discriminating between melanins and lipofuscins in paraffin sections of animal tissue

General Description

Nile Blue A is aminodiethylaminonaphthophenoxazonium sulfate employed in biology and histology. It is a highly fluorescent, and photostable organic dye used to dye clothing, and is for use in lipid histochemistry.

Biochem/physiol Actions

Nile blue A stains acidic components, such as phospholipids, nucleic and fatty acids by imparting a dark blue color, while neutral lipids, namely triglycerides, are stained pink, or red.

Purification Methods

Crystallise the dye from aqueous AcOH. It has UV: at 630nm (96% aqueous max EtOH) and 635nm (H2O). The betaine has UV: at 513nm (EtOH). [Crossley et al. J Am Chem Soc 74 max 57, 578 1952, Merill & Spencer J Am Chem Soc 70 3683 1948, Beilstein 27 II 457, 27 III/IV 5166.]

CI 51180Supplier

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