CI 51180 Chemical Properties

Melting point:

>300 °C (dec.)(lit.)

storage temp. 

room temp

solubility 

Soluble in water, ethanol

Colour Index 

51180

form 

Powder

color 

Dark green to black

PH Range

10.1(BLUE)-11.1(RED)

Water Solubility 

Soluble in ethanol, and water (50 g/L) at 25°C.

λmax

633 nm

Biological Applications

Detecting microorganisms; treating virus infectious diseases; photodynamic therapy

Safety Information

Hazard Codes 

Xi

Safety Statements 

22-24/25

WGK Germany 

3

RTECS 

DJ1931000

HS Code 

3212900000

Toxicity

mouse,LD,intraperitoneal,> 500mg/kg (500mg/kg),"Summary Tables of Biological Tests," National Research Council Chemical-Biological Coordination Center. Vol. 4, Pg. 46, 1952.

MSDS

• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

CI 51180 Usage And Synthesis

Chemical Properties

dark green to black powder

Uses

Nile Blue A is used in the Lillie and Fullmer method for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue. Nile blue A is also useful for staining fats and phospholipids. Dyes and metabolites.

Uses

Nile blue A is suitable for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue, and in a histochemical method for lipids in frozen sections.

Uses

Nile Blue A is a stain used for discriminating between melanins and lipofuscins in paraffin sections of animal tissue

General Description

Nile Blue A is aminodiethylaminonaphthophenoxazonium sulfate employed in biology and histology. It is a highly fluorescent, and photostable organic dye used to dye clothing, and is for use in lipid histochemistry.

Biochem/physiol Actions

Nile blue A stains acidic components, such as phospholipids, nucleic and fatty acids by imparting a dark blue color, while neutral lipids, namely triglycerides, are stained pink, or red.

Purification Methods

Crystallise the dye from aqueous AcOH. It has UV: at 630nm (96% aqueous max EtOH) and 635nm (H2O). The betaine has UV: at 513nm (EtOH). [Crossley et al. J Am Chem Soc 74 max 57, 578 1952, Merill & Spencer J Am Chem Soc 70 3683 1948, Beilstein 27 II 457, 27 III/IV 5166.]

CI 51180(3625-57-8)Related Product Information

• o-Cresolphthalein Complexone
• Ammonium iron(III) sulfate
• Bromocresol green
• Trichloroacetic acid
• Sodium dodecylbenzenesulphonate
• DARROW RED CERTIFIED
• Resazurin sodium salt
• ORCEIN
• CRESYL VIOLET ACETATE
• Basic Blue 11
• Pure sulfuric acid
• BASIC BLACK 1
• CI 51180
• 3-Aminophenol hemisulfate
• CI 51180
• 2-Aminophenol hemisulfate
• 2,5-Diaminoanisole sulfate
• CI-51180