Rhodamine B Chemical Properties

Melting point:

210-211 (dec.)(lit.)

Density 

0.79 g/mL at 20 °C

refractive index 

1.6500 (estimate)

Flash point:

12 °C

storage temp. 

room temp

solubility 

H2O: soluble1mg/mL

form 

Solid

Colour Index 

45170

color 

Green

PH

3-4 (10g/l, H2O, 20℃)

Water Solubility 

SOLUBLE

Merck 

14,8183

BRN 

4119648

Stability:

Stable. Incompatible with strong oxidizing agents.

LogP

1.9-2

CAS DataBase Reference

81-88-9(CAS DataBase Reference)

NIST Chemistry Reference

Rhodamine b(81-88-9)

IARC

3 (Vol. 16, Sup 7) 1987

EPA Substance Registry System

Rhodamine B (81-88-9)

Safety Information

Hazard Codes 

Xn,Xi,F

Risk Statements 

22-41-68-67-36-11-40-20/21/22-52/53

Safety Statements 

7-16-24/25-26-36/37/39-39-36-22

WGK Germany 

3

RTECS 

BP3675000

TSCA 

Yes

HS Code 

32041300

Hazardous Substances Data

81-88-9(Hazardous Substances Data)

Toxicity

LD50 i.v. in rats: 89.5 mg/kg (Webb)

MSDS

• Language:English Provider:Rhodamine B
• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

Rhodamine B Usage And Synthesis

Description

C.I. Food red 15 is a green crystalline or redviolet powdered solid. Molecular weight =479.1. HazardIdentification (based on NFPA-704 M Rating System):Health 1, Flammability 1, Reactivity 0. Highly soluble inwater.

Chemical Properties

red/brown or green crystals

Chemical Properties

C.I. Food red 15 is a green crystalline or redviolet powdered solid

Uses

A useful fluorochrome for histology, FRET and mitochondrial probe.

Uses

As a dye, especially for paper; as a reagent for antimony, bismuth, cobalt, niobium, gold, manganese, mercury, molybdenum, tantalum, thallium, tungsten; as biological stain. Provisionally listed for use in drugs and cosmetics.

Uses

Rhodamine B is used in biological studies for an anti-reactive oxygen species/hepatic fibrosis drug delivery system based on salvianolic acid B loaded mesoporous silica nanoparticles. Dyes and metabolites, Environmental Testing.

Definition

ChEBI: An organic chloride salt having N-[9-(2-carboxyphenyl)-6-(diethylamino)-3H-xanthen-3-ylidene]-N-ethylethanaminium as the counterion. An amphoteric dye commonly used as a fluorochrome.

General Description

Green crystals or reddish-violet powder. Used as a dye, especially for paper, as a metal chelating reagent, and in drugs and cosmetics.

Air & Water Reactions

Very soluble in water. Solution is bluish-red. Dilute solutions are strongly fluorescent.

Reactivity Profile

Acidic organic/inorganic salts, such as Rhodamine B, are generally soluble in water. The resulting solutions contain moderate concentrations of hydrogen ions and have pH's of less than 7.0. They react as acids to neutralize bases. These neutralizations generate heat, but less or far less than is generated by neutralization of inorganic acids, inorganic oxoacids, and carboxylic acid. They usually do not react as either oxidizing agents or reducing agents but such behavior is not impossible. Many of these compounds catalyze organic reactions.

Hazard

Questionable carcinogen.

Potential Exposure

It is used as a color additive in drugs, foods, cosmetics, and fabric dyes. It is also used as a tracing agent in water pollution studies. May be used as an agricultural chemical

First aid

If this chemical gets into the eyes, remove anycontact lenses at once and irrigate immediately for at least15 min, occasionally lifting upper and lower lids. Seek medical attention immediately. If this chemical contacts theskin, remove contaminated clothing and wash immediatelywith soap and water. Seek medical attention immediately. If this chemical has been inhaled, remove from exposure,begin rescue breathing (using universal precautions, including resuscitation mask) if breathing has stopped and CPR ifheart action has stopped. Transfer promptly to a medicalfacility. When this chemical has been swallowed, get medical attention. Give large quantities of water and inducevomiting. Do not make an unconscious person vomit.

storage

Color Code—Blue: Health Hazard/Poison: Storein a secure poison location. Prior to working with C.I. Foodred 15 you should be trained on its proper handling andstorage. Store in tightly closed containers in a cool, wellventilated area away from oxidizers and reducing agents.Where possible, automatically pump liquid from drums orother storage containers to process containers.

Shipping

UN3143 Dyes, solid, toxic, n.o.s. or Dye intermediates, solid, toxic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required.

Properties and Applications

TEST ITEMS	SPECIFICATION
APPEARANCE	GREEN GLITTERY CRYSTAL
SHADE (COMPARE TO STANDARD)	CLOSE
WATER SOLUBILITY (60°C)	8 g/L min
WATER INSOLUBLE	0.5% max
TINTING STRENGTH	500-505 %

Purification Methods

Major impurities are partially dealkylated compounds not removed by recrystallisation.Purify the dye by chromatography, using ethyl acetate/isopropanol/ammonia (conc)(9:7:4, RF 0.75 on Kieselgel G). It has also been crystallised from a concentrated solution in MeOH by slow addition of dry diethyl ether; or from EtOH containing a drop of conc HCl by slow addition of ten volumes of dry diethyl ether. The solid is washed with ether and air dried. The dried material has also been extracted with *benzene to remove oil-soluble material prior to recrystallisation. Store it in the dark. [Beilstein 18 II 486, 18 III/IV 8246, 19/8 V 669.]

Incompatibilities

Dust may form explosive mixture with air. Incompatible with oxidizers (chlorates, nitrates, peroxides, permanganates, perchlorates, chlorine, bromine, fluorine, etc.); contact may cause fires or explosions. Keep away from alkaline materials, strong bases, strong acids, oxoacids, epoxides, strong reducing agents

Rhodamine B(81-88-9)Related Product Information

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