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MES monohydrate

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MES monohydrate Basic information

Product Name:
MES monohydrate
Synonyms:
  • 2-(4-morpholinyl)ethanesulfonic acid hydrate
  • MES monohydrate, ≥99% (T)
  • 2-(N-MORPHOLINO)ETHANESULFONIC ACID, MONOHYDRATE2-(N-MORPHOLINO)ETHANESULFONIC ACID, MONOHYDRATE2-(N-MORPHOLINO)ETHANESULFONIC ACID, MONOHYDRATE2-(N-MORPHOLINO)ETHANESULFONIC ACID, MONOHYDRATE
  • MES 1HYD FREE ACID XTL
  • MES FREE ACID MONOHYDRATE ULTRA PURE GRADE 99+% 2-MORPHOLINOETHANESULFONIC ACID
  • Mes-H2-(N-Morpholino)EthanesulfonicAcidMonohydrate
  • MesBuffer,Monohydrate
  • MESH2OMESmonohydrate
CAS:
145224-94-8
MF:
C6H15NO5S
MW:
213.25
EINECS:
224-632-3
Product Categories:
  • Buffer
Mol File:
145224-94-8.mol
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MES monohydrate Chemical Properties

Melting point:
>300 °C(lit.)
storage temp. 
Sealed in dry,Room Temperature
solubility 
H2O: 0.5 M at 20 °C, clear
form 
Powder, Crystals or Flakes
color 
White to pale yellow and/or orange
PH Range
5.5 - 6.7
Odor
Odorless
PH
2.5-4.0 (25℃, 0.5M in H2O)
pka
6.1(at 25℃)
Water Solubility 
Soluble in water.
λmax
λ: 260 nm Amax: 0.025
λ: 280 nm Amax: 0.020
Merck 
14,5902
BRN 
141319
InChI
InChI=1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
InChIKey
MIIIXQJBDGSIKL-UHFFFAOYSA-N
SMILES
S(=O)(=O)(O)CCN1CCOCC1.O
CAS DataBase Reference
145224-94-8(CAS DataBase Reference)
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Safety Information

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-36-37/39
WGK Germany 
3
RTECS 
QE3479500
TSCA 
Yes
HS Code 
29349990

MSDS

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MES monohydrate Usage And Synthesis

Description

MES Monohydrate is a zwitterionic buffer that is not absorbed through cell membranes and is virtually transparent in UV light. MES is a buffering agent used in many biological and biochemical applications. It is also used as a running buffer for denaturing gel electrophoresis. The characteristics of low UV absorptivity, minimal reactivity, stable pH and solubility in water allow MES Monohydrate to be used as a Good's buffer.

Chemical Properties

white crystalline powder

Uses

MES Monohydrate has been used:

  • To adjust the pH of growth medium to 5.5-7.0 in an in vitro cell wall stress assay
  • As a buffering agent to stabilize enzymatic solutions
  • As a component of PAGE running buffer

Uses

MES monohydrate is a biological buffer often referred to as Good?s buffer. It is useful for cell culture media and protein based buffer formulations to maintain a stable environment in solution. It is also used in the purification bioprocesses of antibodies, peptides, proteins, blood components and growth factors. It is a useful resolving agent for very small proteins on bis-tris gels. Buffer solutions are used as a means of keeping pH at a nearly constant value in a wide variety of chemical applications.

Uses

MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography. MES is often employed as a substitute for the highly toxic cacodylate, as well as a substitute for the non-zwitterionic buffers citrate and malate. MES complexes with some organotin(IV) molecules that are being explored as antitumor agents.
MES free acid or MES free acid monohydrate can be mixed with MES sodium salt to acquire desired pH. Alternatively, MES free acid or MES free acid monohydrate can be titrated with sodium hydroxide to attain desired pH.

Application

MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in biochemical and biological techniques where highly purified products are needed.
MES Monohydrate has been used:
To adjust the pH of growth medium to 5.5-7.0 in an in vitro cell wall stress assay.
As a buffering agent to stabilize enzymatic solutions.
As a component of PAGE running buffer.

General Description

buffer substance MES

Preparation and handling

A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Precautions

Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unkown.

MES monohydrateSupplier

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