Bromelain from pineapple stem 2 u/mg Chemical Properties

storage temp. 

-20°C

solubility 

H2O: soluble1.0mg/mL, clear, colorless

form 

powder

color 

yellow-brown

Safety Information

Hazard Codes 

Xn,Xi

Risk Statements 

36/37/38-42

Safety Statements 

22-24-26-36/37-36

WGK Germany 

1

F 

10-21

HS Code 

35079090

Bromelain from pineapple stem 2 u/mg Usage And Synthesis

Description

Bromelain is a major sulfhydryl proteolytic enzyme found in pineapple plants (Ananas comosus (L.) Merryl). Bromelain is usually found in the fruit and stem, but it is also easy to find in small amounts in the leaf, skin, core, etc. Bromelain from pineapple stem is known as stem bromelain (SBM). SBM has a unique content compared with bromelain from fruit (FBM) because SBM contains many mixtures of various types of thiol-endopeptidases. Based on the research of Heinecke and Gortner, SBM concentrations are better than FBM. Bromelain has been reported to have medical efficacy. Bromelain has been used traditionally to relieve tense muscles, inflammation, and connective tissue. In recent years, bromelain has been used as an anti-inflammatory, antiedematous, and antithrombotic agent (preventing blood clotting). In addition, bromelain showed activity in preventing lung metastases and potentially as an anticancer agent[1].

Chemical Properties

Yellow-brown powder

Uses

Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .

Biochem/physiol Actions

Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.

Purification Methods

This protease has been purified via the acetone powder, G-75 Sephadex gel filtration and Bio-Rex 70 ion-exchange chromatography, and has  1cm 20.1 at 280nm. The protease from pineapple hydrolyses benzoyl glycine ethyl ester with a Km (app) of 210mM and kcat of 0.36 sec-1 . [Murachi Methods Enzymol 19 273 1970, Balls et al. Ind Eng Chem 33 950 1941.]

References

[1] Khairunnisa, F. A. et al. “Bromelain content of extract from stem of pineapple (Ananas comosus (L.) Merr).” 2018. 0.

Bromelain from pineapple stem 2 u/mg(37189-34-7)Related Product Information

• DISPASE
• N/A
• MG-132
• CHYMOPAPAIN
• flavourzyme
• EC 3.4.21.19
• Collagenase
• CATHEPSIN H
• EC 3.4.21.14
• Proteinase K
• Urinary Trypsin Inhibitor
• DISPASE
• ACHROMOPEPTIDASE
• Trypsin
• MG-115
• trysin-chymotrypsin
• Basic protease
• Bromelain