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Ponceau S

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Ponceau S Basic information

Product Name:
Ponceau S
Synonyms:
  • 2,7-Naphthalenedisulfonicacid,3-hydroxy-4-[[2-sulfo-4-[(4-sulfophenyl)azo]phenyl]azo]-,tetrasodiumsalt
  • c.i.acidred112
  • PONCEAU S, SODIUM SALT
  • PONCEAU S
  • LABOTEST-BB LT00847573
  • PONCEAU S (C.I. 27195) FOR MICROSCOPY CE
  • ATX PONCEAU S RED
  • FAST PONCEAU 2B
CAS:
6226-79-5
MF:
C22H17N4NaO13S4
MW:
696.62
EINECS:
228-319-2
Product Categories:
  • P
  • Stains and Dyes
  • Organics
  • Stains&Dyes, A to
Mol File:
6226-79-5.mol
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Ponceau S Chemical Properties

Density 
1.01 g/mL at 20 °C
refractive index 
n20/D 1.338
storage temp. 
Store at RT.
solubility 
H2O: soluble1mg/mL
form 
Powder/Solid
Colour Index 
27195
color 
Red-brown
PH
9.0 (10g/l, H2O, 20℃)
Water Solubility 
10 g/L
Stability:
Stable. Incompatible with strong oxidizing agents.
CAS DataBase Reference
6226-79-5(CAS DataBase Reference)
EPA Substance Registry System
2,7-Naphthalenedisulfonic acid, 3-hydroxy-4-[[2-sulfo-4-[(4-sulfophenyl)azo]phenyl]azo]-, tetrasodium salt (6226-79-5)
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Safety Information

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-36-37/39
WGK Germany 
3
RTECS 
QJ6600000
TSCA 
Yes
HS Code 
32041200

MSDS

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Ponceau S Usage And Synthesis

Chemical Properties

red powder

Uses

Ponceau S dye is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It is also suitable for electrophoresis, azo dye for reversible protein staining. This stain can also be used as a fiducial marker in mass spectrometric imaging.

Definition

ChEBI: An organic sodium salt that is the tetrasodium salt of 3-hydroxy-4-({2-sulfo-4-[(4-sulfophenyl)diazenyl]phenyl}diazenyl)naphthalene-2,7-disulfonic acid.

Preparation

3,4′-Disulfo-4-aininoazobenzene diazo, coupled with 3-Hydroxynaphthalene-2,7-disulfonic acid.

storage

Room temperature

Properties and Applications

colourful red blue light. Soluble in water for product red. The strong sulfuric acid in blue, diluted into orange red. Add in aqueous solution hydrochloric acid basic color; Add sodium hydroxide, thicker solution will appear precipitation.

Standard Light Fastness Soaping Persperation Fastness Oxygen bleaching Fastness to seawater
Fading Stain Fading Stain Fading Stain
ISO 3-4 2-3 3-4 2 1-2 3

Ponceau SSupplier

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