Y27632 (hydrochloride) Chemical Properties

Melting point:

258℃

storage temp. 

2-8°C

solubility 

H2O: soluble14mg/mL

form 

powder

color 

white to beige

Stability:

Stable for 2 years from date of purchase as supplied. Solutions in DMSO or methanol may be stored at -20° for up to 3 months.

Safety Information

Hazard Codes 

Xn

Risk Statements 

20/21/22

Safety Statements 

36

WGK Germany 

3

HS Code 

29333990

Y27632 (hydrochloride) Usage And Synthesis

Description

Y-27632 dihydrochloride (129830-38-2) is a specific inhibitor of ROCK family kinases.1 Enhances stem cell survival and proliferation in culture.2 Y-27632 dihydrochloride significantly improves freeze/thaw survival rate for human embryonic stem cells without influencing morphology, karyotype, cell surface markers, or differentiation potential.3

Uses

A cell permeable inhibitor of ROCK-1 and ROCK-2

Uses

Y-27632 dihydrochloride has been used:

• as a medium supplement in pancreatic ductal adenocarcinoma organoid culture
• in the inhibition of Ras homolog gene family (Rho) kinase in mouse embryonic stem cells
• in the inhibition of Rho-associated protein kinase (ROCK) in human embryonic stem cells and human induced pluripotent stem cells (iPSCs)

General Description

Y-27632 dihydrochloride is an effective bronchodilator and improves lung resistance (RL) induced by antigen and acetylcholine. Y-27632 controls neutrophil migration and lung edema and may be a potential drug for treating acute lung injury.

Biochem/physiol Actions

Y-27632 is highly potent, cell-permeable, selective ROCK (Rho-associated coiled coil forming protein serine/threonine kinase) inhibitor. Ki = 140 nM for p160ROCK. Y-27632 also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP.

storage

Desiccate at RT

References

1) Ishizaki et al. (2000), Pharmacological Properties of Y-27632, a Specific Inhibitor of Rho-Associated Kinases; Mol. Pharmacol., 57 976 2) Gauthaman et al. (2010), Effects of ROCK Inhibitor Y-27632 on Normal and Variant Human Embryonic Stem Cells (hESCs) In Vitro: Its Benefits in hESC Expansion; Stem Cell Rev., 6 86 3) Li et al. (2008), The ROCK Inhibitor Y-27632 enhances the Survival Rate of Human Embryonic Stem Cells Following Cryopreservation; Stem Cells Dev., 17 1079 4) Morel et al. (2018), Proteomics Reveals Scope of Mycolactone-mediated Sec61 Blockade and Distinctive Stress Signature Y-27632; Mol. Cell. Proteomics, 17 1750 [Focus Citation] 5) Yamazaki et al. (2020) Chromatin condensation retains the osteogenic transcription factor, RUNX2, in the nucleus of human mesenchymal stem cells; J. Biomechanical Science and Engineering 15? 1 [Focus Citation]

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