Y27632 (hydrochloride) Chemical Properties

Melting point:

258℃

storage temp. 

2-8°C

solubility 

H2O: soluble14mg/mL

form 

powder

color 

white to beige

biological source

synthetic (organic)

optical activity

[α]/D +3.0 to +5.0°, c =1.0 in methanol

Water Solubility 

H2O: 14mg/mL

Stability:

Stable for 2 years from date of purchase as supplied. Solutions in DMSO or methanol may be stored at -20° for up to 3 months.

InChI

InChI=1/C14H21N3O.2ClH/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13;;/h6-12H,2-5,15H2,1H3,(H,16,17,18);2*1H/t10-,11?,12?;;/s3

InChIKey

IDDDVXIUIXWAGJ-MEIAXHMCNA-N

SMILES

C1(C(NC2=CC=NC=C2)=O)CCC([C@H](N)C)CC1.[H]Cl.[H]Cl |&1:13,r|

Safety Information

Hazard Codes 

Xn

Risk Statements 

20/21/22

Safety Statements 

36

WGK Germany 

3

HS Code 

29333990

Y27632 (hydrochloride) Usage And Synthesis

Description

Y-27632 dihydrochloride (129830-38-2) is a specific inhibitor of ROCK family kinases.1 Enhances stem cell survival and proliferation in culture.2 Y-27632 dihydrochloride significantly improves freeze/thaw survival rate for human embryonic stem cells without influencing morphology, karyotype, cell surface markers, or differentiation potential.3

Uses

A cell permeable inhibitor of ROCK-1 and ROCK-2

Uses

Y-27632 dihydrochloride has been used:

• as a medium supplement in pancreatic ductal adenocarcinoma organoid culture
• in the inhibition of Ras homolog gene family (Rho) kinase in mouse embryonic stem cells
• in the inhibition of Rho-associated protein kinase (ROCK) in human embryonic stem cells and human induced pluripotent stem cells (iPSCs)

General Description

Y-27632 dihydrochloride is an effective bronchodilator and improves lung resistance (RL) induced by antigen and acetylcholine. Y-27632 controls neutrophil migration and lung edema and may be a potential drug for treating acute lung injury.

Biochem/physiol Actions

Y-27632 is highly potent, cell-permeable, selective ROCK (Rho-associated coiled coil forming protein serine/threonine kinase) inhibitor. Ki = 140 nM for p160ROCK. Y-27632 also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP.

storage

Desiccate at RT

References

[1] T ISHIZAKI. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases.[J]. Molecular Pharmacology, 2000, 57 5: 976-983.
[2] KALAMEGAM GAUTHAMAN  Ariff B  Chui Yee Fong. Effect of ROCK inhibitor Y-27632 on normal and variant human embryonic stem cells (hESCs) in vitro: its benefits in hESC expansion.[J]. Stem Cell Reviews and Reports, 2010, 6 1: 86-95. DOI:10.1007/s12015-009-9107-8
[3] XIANGYUN LI. The ROCK inhibitor Y-27632 enhances the survival rate of human embryonic stem cells following cryopreservation.[J]. Stem cells and development, 2008, 17 6: 1079-1085. DOI:10.1089/scd.2007.0247
[4] JEAN-DAVID MOREL. Proteomics Reveals Scope of Mycolactone-mediated Sec61 Blockade and Distinctive Stress Signature.[J]. Molecular & cellular proteomics : MCP, 2018: 1750-1765. DOI:10.1074/mcp.ra118.000824
[5] M. YAMAZAKI H M H Fujie. Chromatin condensation retains the osteogenic transcription factor, RUNX2, in the nucleus of human mesenchymal stem cells[J]. Journal of Biomechanical Science and Engineering, 1900, 1 1. DOI:10.1299/jbse.20-00083

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