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PR-619

Basic information Safety Supplier Related

PR-619 Basic information

Product Name:
PR-619
Synonyms:
  • PR-619
  • 2,6-DiaMino-3,5-dithiocyanopyridine
  • 3,5-dithiocyanatopyridine-2,6-diaMine
  • Thiocyanic acid, C,C'-(2,6-diamino-3,5-pyridinediyl) ester
  • 2,6-Diamino-3,5-dithiocyanopyridine PR 619
  • PR 619 2,6-Diamino-3,5-dithiocyanopyridine
  • 2,6-Diaminopyridine-3,5-bis(thiocyanate)
  • PR-619;PR619
CAS:
2645-32-1
MF:
C7H5N5S2
MW:
223.28
EINECS:
200-256-5
Product Categories:
  • Inhibitors
Mol File:
2645-32-1.mol
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PR-619 Chemical Properties

Melting point:
210℃
Boiling point:
406.0±45.0 °C(Predicted)
Density 
1.61±0.1 g/cm3(Predicted)
storage temp. 
-20°C
solubility 
DMSO: soluble5mg/mL (clear solution)
pka
-0.19±0.50(Predicted)
form 
powder
color 
white to beige
Stability:
Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 1 month.
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Safety Information

Hazard Codes 
Xn
Risk Statements 
22-37/38-41-43
Safety Statements 
26-36/37/39
WGK Germany 
3
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PR-619 Usage And Synthesis

Description

PR-619 (2645-32-1) is a nonselective, reversible inhibitor of cysteine-reactive deubiquitinating enzymes (DUBs; 5-20 μM)1. PR-619 is highly useful for preserving ubiquitinated proteins during cell lysis. DUBs become unregulated in cell lysates and will act quickly to deubiquitinate proteins of interest during cell lysis. To preserve maximum amounts of ubiquitinated protein, include PR-619 (50-100 μM) in lysis buffers. PR-619 is also useful for interrogating the role of DUBs in intact cell systems2, and treatment of cells results in an overall accumulation of ubiquitinated proteins and many other phenotypes.

Uses

PR 619 is a broad-range DUB inhibitor. A selective and general inhibitior of cellular DUB activity without direct impairment of proteasomal proteolysis. Used in the preparation of thiazolopyridines an d bisthiazolopyridines.

Uses

PR-619 has been used:

  • as a component of lysis buffer and also as a deubiquitinase inhibitor to treat proteins derived from SILAC-labeled Jurkat cells
  • as a pan-deubiquitinase inhibitor to study its effect on adenoassociated virus (AAV) transduction
  • in radioimmunoprecipitation assay buffer (RIPA) buffer used for ubiquitination assays

Biochem/physiol Actions

PR-619 is a cell permeable broad spectrum deubiquitylating enzymes (DBUs) inhibitor. PR-619 induces the accumulation of polyubiquitylated proteins in cells without directly affecting proteasome activity.

storage

Store at -20°C

References

1) Altun?et al. (2011),?Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes; Chem. Biol.,?18?1401 2) Seiberlich?et al. (2012),?The small molecule inhibitor PR-619 of deubiquitinating enzymes affects the microtubule network and causes protein aggregate formation in neural cells: implications for neurodegenerative diseases; Biochim. Biophys. Acta,?1823?2057

PR-619Supplier

Shanghai Boyle Chemical Co., Ltd.
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J & K SCIENTIFIC LTD.
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010-82848833 400-666-7788
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jkinfo@jkchemical.com
Jinan Trio PharmaTech Co., Ltd.
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+86 (531) 88811783
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sales@trio-pharmatech.com (International market)
Dalian Meilun Biotech Co., Ltd.
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0411-62910999 13889544652
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sales@meilune.com
Shanghai T&W Pharmaceutical Co., Ltd.
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+86 21 61551611