Hoechst 33258 analog 2 Chemical Properties

Boiling point:

712.4±70.0 °C(Predicted)

Density 

1.276±0.06 g/cm3(Predicted)

storage temp. 

Sealed in dry,Store in freezer, under -20°C

solubility 

25℃: DMSO or water Protect from light

form 

Powder

pka

10.89±0.10(Predicted)

color 

Off-white to pink

Appearance

White to Light Yellow Crystalline Solid

Hoechst 33258 analog 2 Usage And Synthesis

Description

Hoechst 33258 analog 2 is a blue fluorescent dye used for staining DNA.

Uses

Hoechst 33258 analog 2 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution[1].

References

[1] Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505. DOI:10.1177/23.7.1095650
[2] a b c "Hoechst Stains". Invitrogren (Molecular Probes).
[3] Portugal J, Waring MJ. Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68. DOI:10.1016/0167-4781(88)90079-6