Sulfo-NHS-Biotin Chemical Properties

storage temp. 

-20°C

solubility 

H₂O: 10 mg/mL

form 

powder

CAS DataBase Reference

119616-38-5(CAS DataBase Reference)

Safety Information

Hazard Codes 

Xn

Risk Statements 

20/21/22-36/37/38-63

Safety Statements 

22-26-36

WGK Germany 

3

HS Code 

29349990

MSDS

• Language:English Provider:SigmaAldrich

Sulfo-NHS-Biotin Usage And Synthesis

Description

Sulfo-NHS-Biotin is a linker containing a sulfo-NHS moiety and a biotin group. Sulfo-NHS enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates for conjugation with primary amines. The terminal biotinylation can react with amine molecules in the presence of activator EDC or HATU.

Chemical Properties

1.3

Uses

apoptosis probe, cell permeable fluorescent Ca2+-sensitive dye for cystolic Ca2+ assays

Uses

Sulfo-NHS-Biotin is a biotinylation reagent used for biotinylating antibody or protein that can then be used for protein detection or immobilization studies when used in conjunction with streptavidin and immobilized streptavidin. Sulfo-NHS-Biotin was shown to work most effectively when targeting cell surface proteins.

General Description

Biotin:avidin second antibody signal systems involve the conjugation of biotin to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin which has been conjugated to an enzyme or signal molecule to bind to the antibody associated biotin molecule.

Biological Activity

sulfo-nhs-biotin (n-hydroxysulfosuccinimidobiotin) is an amine-reactive biotinylation reagent. the most common amine-reactive biotinylation reagents can be divied into two basic types, n-hydroxysuccinimide (nhs) esters and carboxylates. generally, nhs esters biotinylation reagent contains a reactive nhs ring structure that reacts with an amine on the carbonyl group of a protein or other molecules through nucleophilic attack subsequently forming a stable amide linkage and releasing the nhs group. due to the insolubility of nhs-biotin in aqueous environments, sulfo-nhs-biotin, a water-soluble analog of nhs-biotin, has been developed by attaching a negatively charged sulfonate group on the nhs ring structure, which can be added directly to aqueous reactions without the need for organic solvent dissolution.bioconjugate techniques , 2nd ed. by greg t.hermanson (pierce biotechnology, thermo fisher scientific, rockford, il). academic press (an imprint of elsevier): london, amsterdam, burlington, san diego . 2008. isbn 978-0-12-370501-3.

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