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EOSIN B

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EOSIN B Basic information

Product Name:
EOSIN B
Synonyms:
  • C.I. Acid Red 91
  • Eosin B, high purity biological stain
  • Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 4',5'-dibromo-3',6'-dihydroxy-2',7'-dinitro-, disodium salt
  • 2-(4,5-dibromo-3,6-dihydroxy-2,7-dinitroxanthen-9-yl)-benzoic acid, disodium salt
  • EOSIN B CERTIFIED
  • EOSIN B CERTIFIED (C.I. 45400)
  • EOSIN SCARLET, FOR MICROSCOPY
  • EOSIN BLUISH, WATER-SOLUBLE, PURE, FOR M ICROSCOPY
CAS:
548-24-3
MF:
C20H9Br2N2NaO9
MW:
604.09
EINECS:
208-943-1
Product Categories:
  • Organics
  • Xanthene
Mol File:
548-24-3.mol
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EOSIN B Chemical Properties

Melting point:
275 °C (dec.)(lit.)
storage temp. 
Store at +5°C to +30°C.
solubility 
spirit: soluble
Colour Index 
45400
form 
Solid
color 
Very dark purple-red to dark brown
PH
6.2 (10g/l, H2O, 25℃)
Water Solubility 
Soluble in water (390 g/L at 20°C), and ethanol.
λmax
514 nm, 395 nm
Merck 
14,3602
BRN 
4121964
Stability:
Stable. Incompatible with strong oxidizing agents.
Biological Applications
Antimalarial agent; protein assay; detecting enzyme activity; treating cancer,malaria,diabetes,a variety of conditions affecting skin,mouth,digestive tract,urinary tract,reproductive tract,respiratory tract,circulatory sys
EPA Substance Registry System
Eosin B (548-24-3)
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Safety Information

Hazard Codes 
Xn
Risk Statements 
22
Safety Statements 
22-24/25
WGK Germany 
3
TSCA 
Yes
HS Code 
3204 12 00

MSDS

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EOSIN B Usage And Synthesis

Chemical Properties

odourless green to reddish-brown solid

Uses

Dyeing wool, cotton, and paper. In histology as a stain for epithelia, muscular fibers, nuclei, etc.

Uses

Eosin B sodium salt is a cell and tissue stain. Eosin B sodium salt is used as a counter stain for collagen, as a tissue stain for cell granules, nuclei, and microorganisms when coupled with Azure A (sc-203729), and as a stain for cell differentiation of the anterior pituitary.

Preparation

will 4′,5′-Dibromofluorescein?(C.I. Acid Orange 11, C.I. 45370) nitrification, and translated into sodium salt or ammonium salt.

Definition

ChEBI: An organic sodium salt which is the disodium salt of eosin b diphenol.

Purification Methods

Free it from inorganic halides by repeated crystallisation from butan-1-ol. [Beilstein 19/6 V 469.]

Properties and Applications

colourful red blue light.

Standard Light Fastness Soaping Persperation Fastness Oxygen bleaching Fastness to seawater
Fading Stain Fading Stain Fading Stain
ISO 3 3 3 3 3

EOSIN BSupplier

J & K SCIENTIFIC LTD.
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