EOSIN B
EOSIN B Basic information
- Product Name:
- EOSIN B
- Synonyms:
-
- C.I. Acid Red 91
- Eosin B, high purity biological stain
- Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 4',5'-dibromo-3',6'-dihydroxy-2',7'-dinitro-, disodium salt
- 2-(4,5-dibromo-3,6-dihydroxy-2,7-dinitroxanthen-9-yl)-benzoic acid, disodium salt
- EOSIN B CERTIFIED
- EOSIN B CERTIFIED (C.I. 45400)
- EOSIN SCARLET, FOR MICROSCOPY
- EOSIN BLUISH, WATER-SOLUBLE, PURE, FOR M ICROSCOPY
- CAS:
- 548-24-3
- MF:
- C20H9Br2N2NaO9
- MW:
- 604.09
- EINECS:
- 208-943-1
- Product Categories:
-
- Organics
- Xanthene
- Mol File:
- 548-24-3.mol
EOSIN B Chemical Properties
- Melting point:
- 275 °C (dec.)(lit.)
- bulk density
- 680kg/m3
- storage temp.
- Store at +5°C to +30°C.
- solubility
- spirit: soluble
- Colour Index
- 45400
- form
- Solid
- color
- Very dark purple-red to dark brown
- PH
- 6.2 (10g/l, H2O, 25℃)
- Water Solubility
- Soluble in water (390 g/L at 20°C), and ethanol.
- λmax
- 514 nm, 395 nm
- Merck
- 14,3602
- BRN
- 4121964
- Stability:
- Stable. Incompatible with strong oxidizing agents.
- Biological Applications
- Antimalarial agent; protein assay; detecting enzyme activity; treating cancer,malaria,diabetes,a variety of conditions affecting skin,mouth,digestive tract,urinary tract,reproductive tract,respiratory tract,circulatory sys
- Major Application
- diagnostic assay manufacturing
hematology
histology - InChI
- 1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
- InChIKey
- GYYTYUGGVBYJHE-UHFFFAOYSA-L
- SMILES
- [Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(C(=O)C(Br)=C3Oc4c(Br)c([O-])c(cc24)[N+]([O-])=O)[N+]([O-])=O
- EPA Substance Registry System
- Eosin B (548-24-3)
MSDS
- Language:English Provider:ACROS
- Language:English Provider:SigmaAldrich
- Language:English Provider:ALFA
EOSIN B Usage And Synthesis
Chemical Properties
odourless green to reddish-brown solid
Uses
Eosin B sodium salt is a cell and tissue stain. Eosin B sodium salt is used as a counter stain for collagen, as a tissue stain for cell granules, nuclei, and microorganisms when coupled with Azure A (sc-203729), and as a stain for cell differentiation of the anterior pituitary.
Uses
Dyeing wool, cotton, and paper. In histology as a stain for epithelia, muscular fibers, nuclei, etc.
Definition
ChEBI: An organic sodium salt which is the disodium salt of eosin b diphenol.
Preparation
will 4′,5′-Dibromofluorescein(C.I. Acid Orange 11, C.I. 45370) nitrification, and translated into sodium salt or ammonium salt.
Biological Activity
Eosin B is a xanthene dye and is also referred to as eosin bluish or erythrosin B. It is used in the Harris stain for Negri bodies. Eosin B is also used in Mayer′s Hemalum as a counterstain for collagen. Eosin B-Azure A are used together for the staining of cell granules, nuclei and microorganisms. It can also be used for protein estimation. The protein - eosin B complex absorbs at wavelength range of 536–544nm and the change in blue shift helps in determining the protein concentration.
storage
Room temperature
Purification Methods
Free it from inorganic halides by repeated crystallisation from butan-1-ol. [Beilstein 19/6 V 469.]
Properties and Applications
colourful red blue light.
| Standard | Light Fastness | Soaping | Persperation Fastness | Oxygen bleaching | Fastness to seawater | |||
| Fading | Stain | Fading | Stain | Fading | Stain | |||
| ISO | 3 | 3 | 3 | 3 | 3 | |||
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