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EOSIN B

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EOSIN B Basic information

Product Name:
EOSIN B
Synonyms:
  • C.I. Acid Red 91
  • Eosin B, high purity biological stain
  • Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 4',5'-dibromo-3',6'-dihydroxy-2',7'-dinitro-, disodium salt
  • 2-(4,5-dibromo-3,6-dihydroxy-2,7-dinitroxanthen-9-yl)-benzoic acid, disodium salt
  • EOSIN B CERTIFIED
  • EOSIN B CERTIFIED (C.I. 45400)
  • EOSIN SCARLET, FOR MICROSCOPY
  • EOSIN BLUISH, WATER-SOLUBLE, PURE, FOR M ICROSCOPY
CAS:
548-24-3
MF:
C20H9Br2N2NaO9
MW:
604.09
EINECS:
208-943-1
Product Categories:
  • Organics
  • Xanthene
Mol File:
548-24-3.mol
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EOSIN B Chemical Properties

Melting point:
275 °C (dec.)(lit.)
storage temp. 
Store at +5°C to +30°C.
solubility 
spirit: soluble
Colour Index 
45400
form 
Solid
color 
Very dark purple-red to dark brown
PH
6.2 (10g/l, H2O, 25℃)
Water Solubility 
Soluble in water (390 g/L at 20°C), and ethanol.
λmax
514 nm, 395 nm
Merck 
14,3602
BRN 
4121964
Stability:
Stable. Incompatible with strong oxidizing agents.
Biological Applications
Antimalarial agent; protein assay; detecting enzyme activity; treating cancer,malaria,diabetes,a variety of conditions affecting skin,mouth,digestive tract,urinary tract,reproductive tract,respiratory tract,circulatory sys
EPA Substance Registry System
Eosin B (548-24-3)
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Safety Information

Hazard Codes 
Xn
Risk Statements 
22
Safety Statements 
22-24/25
WGK Germany 
3
TSCA 
Yes
HS Code 
3204 12 00

MSDS

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EOSIN B Usage And Synthesis

Chemical Properties

odourless green to reddish-brown solid

Uses

Eosin B sodium salt is a cell and tissue stain. Eosin B sodium salt is used as a counter stain for collagen, as a tissue stain for cell granules, nuclei, and microorganisms when coupled with Azure A (sc-203729), and as a stain for cell differentiation of the anterior pituitary.

Uses

Dyeing wool, cotton, and paper. In histology as a stain for epithelia, muscular fibers, nuclei, etc.

Definition

ChEBI: An organic sodium salt which is the disodium salt of eosin b diphenol.

Preparation

will 4′,5′-Dibromofluorescein?(C.I. Acid Orange 11, C.I. 45370) nitrification, and translated into sodium salt or ammonium salt.

storage

Room temperature

Properties and Applications

colourful red blue light.

Standard Light Fastness Soaping Persperation Fastness Oxygen bleaching Fastness to seawater
Fading Stain Fading Stain Fading Stain
ISO 3 3 3 3 3

Purification Methods

Free it from inorganic halides by repeated crystallisation from butan-1-ol. [Beilstein 19/6 V 469.]

EOSIN BSupplier

J & K SCIENTIFIC LTD.
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