EOSIN B
EOSIN B Basic information
- Product Name:
- EOSIN B
- Synonyms:
-
- C.I. Acid Red 91
- Eosin B, high purity biological stain
- Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 4',5'-dibromo-3',6'-dihydroxy-2',7'-dinitro-, disodium salt
- 2-(4,5-dibromo-3,6-dihydroxy-2,7-dinitroxanthen-9-yl)-benzoic acid, disodium salt
- EOSIN B CERTIFIED
- EOSIN B CERTIFIED (C.I. 45400)
- EOSIN SCARLET, FOR MICROSCOPY
- EOSIN BLUISH, WATER-SOLUBLE, PURE, FOR M ICROSCOPY
- CAS:
- 548-24-3
- MF:
- C20H9Br2N2NaO9
- MW:
- 604.09
- EINECS:
- 208-943-1
- Product Categories:
-
- Organics
- Xanthene
- Mol File:
- 548-24-3.mol
EOSIN B Chemical Properties
- Melting point:
- 275 °C (dec.)(lit.)
- bulk density
- 680kg/m3
- storage temp.
- Store at +5°C to +30°C.
- solubility
- spirit: soluble
- Colour Index
- 45400
- form
- Solid
- color
- Very dark purple-red to dark brown
- PH
- 6.2 (10g/l, H2O, 25℃)
- Water Solubility
- Soluble in water (390 g/L at 20°C), and ethanol.
- λmax
- 514 nm, 395 nm
- Merck
- 14,3602
- BRN
- 4121964
- Stability:
- Stable. Incompatible with strong oxidizing agents.
- Biological Applications
- Antimalarial agent; protein assay; detecting enzyme activity; treating cancer,malaria,diabetes,a variety of conditions affecting skin,mouth,digestive tract,urinary tract,reproductive tract,respiratory tract,circulatory sys
- EPA Substance Registry System
- Eosin B (548-24-3)
MSDS
- Language:English Provider:ACROS
- Language:English Provider:SigmaAldrich
- Language:English Provider:ALFA
EOSIN B Usage And Synthesis
Chemical Properties
odourless green to reddish-brown solid
Uses
Eosin B sodium salt is a cell and tissue stain. Eosin B sodium salt is used as a counter stain for collagen, as a tissue stain for cell granules, nuclei, and microorganisms when coupled with Azure A (sc-203729), and as a stain for cell differentiation of the anterior pituitary.
Uses
Dyeing wool, cotton, and paper. In histology as a stain for epithelia, muscular fibers, nuclei, etc.
Definition
ChEBI: An organic sodium salt which is the disodium salt of eosin b diphenol.
Preparation
will 4′,5′-Dibromofluorescein?(C.I. Acid Orange 11, C.I. 45370) nitrification, and translated into sodium salt or ammonium salt.
Biological Activity
Eosin B is a xanthene dye and is also referred to as eosin bluish or erythrosin B. It is used in the Harris stain for Negri bodies. Eosin B is also used in Mayer′s Hemalum as a counterstain for collagen. Eosin B-Azure A are used together for the staining of cell granules, nuclei and microorganisms. It can also be used for protein estimation. The protein - eosin B complex absorbs at wavelength range of 536–544nm and the change in blue shift helps in determining the protein concentration.
storage
Room temperature
Properties and Applications
colourful red blue light.
| Standard | Light Fastness | Soaping | Persperation Fastness | Oxygen bleaching | Fastness to seawater | |||
| Fading | Stain | Fading | Stain | Fading | Stain | |||
| ISO | 3 | 3 | 3 | 3 | 3 | |||
Purification Methods
Free it from inorganic halides by repeated crystallisation from butan-1-ol. [Beilstein 19/6 V 469.]
EOSIN BSupplier
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