Hoechst 33258 Chemical Properties

Melting point:

314 °C

Density 

1.5099 (rough estimate)

refractive index 

1.6300 (estimate)

storage temp. 

-20°C

solubility 

H₂O: 10 mg/mL

form 

powder

color 

Yellow to Very Dark Yellow

Water Solubility 

SOLUBLE

BRN 

4088183

Stability:

Hygroscopic

CAS DataBase Reference

23491-45-4(CAS DataBase Reference)

EPA Substance Registry System

Phenol, 4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]-, trihydrochloride (23491-45-4)

Safety Information

Hazard Codes 

Xn

Risk Statements 

22-36/38-36/37/38-20/21/22

Safety Statements 

26-36-24/25-23

WGK Germany 

3

RTECS 

SM1140500

F 

3-8-10

HS Code 

32129000

Toxicity

dog,LD,intravenous,> 22400ug/kg (22.4mg/kg),LUNGS, THORAX, OR RESPIRATION: DYSPNEASENSE ORGANS AND SPECIAL SENSES: LACRIMATION: EYEGASTROINTESTINAL: CHANGES IN STRUCTURE OR FUNCTION OF SALIVARY GLANDS,National Technical Information Service. Vol. PB84-171206,

MSDS

• Language:English Provider:p-(5-(5-(4-Methyl-1-piperazinyl)-1H-2-benzimidazolyl)-1H-2-benzimidazolyl)phenol trihydrochloride
• Language:English Provider:SigmaAldrich

Hoechst 33258 Usage And Synthesis

Chemical Properties

yellow to yellow-green powder

Uses

Hoechst 33258 is a Hoechst stain used to stain DNA. It is part of a family of fluorescent dyes. Dyes and metabolites.

Uses

bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry.
Excitation max. =?346 nm
Emission max. =?460 nm

Uses

Hoechst 33258 is useful for staining DNA, chromosomes and nuclei. The nucleic acid binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence

General Description

Membrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca²⁺ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.

Biochem/physiol Actions

bisBenzimide H 33258 is a potent and selective Bcl-XL inhibitor. bisBenzimide H 33258 is a membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA.

storage

+4°C (desiccate)

Hoechst 33258(23491-45-4)Related Product Information

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