CMLD-2 Chemical Properties

storage temp. 

Store at -20°C

solubility 

DMSO: 50 mg/mL (97.36 mM)

form 

Solid

color 

White to off-white

InChIKey

PROGRNRRJJYCNX-UHFFFAOYSA-N

SMILES

C1(=O)OC2=C(C(C3=CC=C(OC)C=C3)CC(=O)N3CCCC3)C(OC)=CC(OC)=C2C(C2=CC=CC=C2)=C1

CMLD-2 Usage And Synthesis

Description

CMLD-2, an inhibitor of HuR-ARE interaction, competitively binds HuR protein, disrupting its interaction with adenine-uridine rich elements (ARE)-containing mRNAs (Ki=350 nM). It is a coumarin-derived small molecule. CMLD-2 induces apoptosis and exhibits antitumor activity in different cancer cells, such as colon, pancreatic, thyroid and lung cancer cell lines. These biological effects of CMLD-2 could be due to the reduced stability of HuR mRNA targets involved in proliferative and anti-apoptotic pathways. Hu antigen R (HuR) is an RNA-binding protein that can regulate target mRNA stability and translation[1].

in vitro

CMLD-2, an inhibitor of HuR-ARE interaction, competitively binds HuR protein, disrupting its interaction with adenine-uridine rich elements (ARE)-containing mRNAs (Ki=350 nM). It is a coumarin-derived small molecule. CMLD-2 induces apoptosis and exhibits antitumor activity in different cancer cells, such as colon, pancreatic, thyroid and lung cancer cell lines. These biological effects of CMLD-2 could be due to the reduced stability of HuR mRNA targets involved in proliferative and anti-apoptotic pathways. Hu antigen R (HuR) is an RNA-binding protein that can regulate target mRNA stability and translation.

References

[1] Lorenzo Allegri. “The HuR CMLD-2 inhibitor exhibits antitumor effects via MAD2 downregulation in thyroid cancer cells.” ACS Applied Electronic Materials (2019): 7374.