BLASTICIDIN S HYDROCHLORIDE Chemical Properties

Melting point:

224-225 °C (decomp)

storage temp. 

−20°C

solubility 

Soluble in Water (greater than 25 mg/ml)

form 

Liquid

color 

White

Water Solubility 

Soluble in water. Insoluble in organic solvents.

Stability:

Stable for 1 year from date of purchase as supplied. Solutions in distilled water may be stored at -20°C for up to 1 month.

InChIKey

YQXYQOXRCNEATG-DQUYGLIRNA-N

SMILES

[C@@H]1(C=C[C@H](NC(=O)C[C@@H](N)CCN(C)C(=N)N)[C@@H](C(=O)O)O1)N1C=CC(N)=NC1=O.Cl |&1:0,3,8,17,r|

Safety Information

Hazard Codes 

T+

Risk Statements 

28

Safety Statements 

28-36/37-45

RIDADR 

UN 2811 6.1/PG 2

WGK Germany 

3

F 

1-10

HazardClass 

6.1

HS Code 

29349990

MSDS

• Language:English Provider:SigmaAldrich

BLASTICIDIN S HYDROCHLORIDE Usage And Synthesis

Description

Blasticidin S HCl (3513-03-9) is a peptidyl nucleoside antibiotic inhibiting protein synthesis in bacteria and eukaryotes.1?Inhibits translation by trapping deformed tRNA on the ribosome.1?The ABC-type transporter NppA1A2BCD is required for uptake of blasticidin S and other peptidyl nucleoside antibiotics in?Pseudomonas aeruginosa?PA14.2?Selection agent for bis, bsr and bsd transformed cells.3,4

Chemical Properties

White powder

Uses

Inhibits protein synthesis and induces apoptosis. Used to study protein synthesis at the level of peptide bond formation.

Uses

Blasticidin S hydrochloride is used to inhibit protein synthesis in prokaryotic and eukaryotic organisms. It also induces apoptosis.

Uses

Blasticidin S hydrochloride is a salt of blasticidin S, an amphoteric nucleoside produced by several species of Streptomyces, first reported in the late 1950s. Blasticidin S hydrochloride was originally isolated fby acetone precipitation. For historical reasons only the salt has been routinely available to researchers. Both the salt and free base are freely water soluble. Blasticidin S is an antifungal agent with particularly potent activity against the rice pathogen, Piricularia oryzae, for which it was used commercially for some time in Japan. Blasticidin S inhibits protein synthesis and is active against bacteria, tumour cell lines and nematodes. More recently, blasticidin S has been used as a marker for strain manipulations.

General Description

Nucleoside antibiotic that specifically inhibits protein synthesis in both prokaryotes and eukaryotes. Suitable for selection of cells carrying plasmids conferring blasticidin resistance. Blasticidin resistance is conferred by the blasticidin S deaminase gene (bsr) that converts blastisidin S to a nontoxic deaminohydroxy derivative.

storage

Store at -20°C

References

[1] EGOR SVIDRITSKIY. Blasticidin S inhibits translation by trapping deformed tRNA on the ribosome.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110 30: 12283-12288. DOI:10.1073/pnas.1304922110
[2] DANIEL PLETZER. The Pseudomonas aeruginosa PA14 ABC Transporter NppA1A2BCD Is Required for Uptake of Peptidyl Nucleoside Antibiotics.[J]. Journal of Bacteriology, 2015, 197 13: 2217-2228. DOI:10.1128/jb.00234-15
[3] MASAHITO ASADA. Transfection of Babesia bovis by Double Selection with WR99210 and Blasticidin-S and Its Application for Functional Analysis of Thioredoxin Peroxidase-1.[J]. PLoS ONE, 2015: e0125993. DOI:10.1371/journal.pone.0125993
[4] ROHINI GARG  Ayub Q. Hemoglobin transforms anti-inflammatory Salmonella typhi virulence polysaccharide into a TLR-2 agonist.[J]. Journal of immunology, 2010, 184 11: 5980-5987. DOI:10.4049/jimmunol.0903512

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