CHOLESTEROL ESTERASE Chemical Properties

vapor pressure 

0.004Pa at 25℃

storage temp. 

-20°C

solubility 

Dissolve 1mg/ml in 0.1M phosphate buffer, pH 6.0 to 7.0

form 

powder

color 

white

EPA Substance Registry System

Esterase, cholesterol (9026-00-0)

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

F 

10-21

TSCA 

Yes

HS Code 

35079090

MSDS

• Language:English Provider:SigmaAldrich

CHOLESTEROL ESTERASE Usage And Synthesis

Chemical Properties

Powder

Uses

Cholesterol Esterase is used for enzymatic determination of total cholesterol when coupled with cholesterol oxidize in clinical analysis. Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols.

Uses

Cholesterol Esterase from porcine pancreas has been used:

• in an in vitro simulated digestion model to improve the hydrolysis of carotenoid esters in the intestinal phase
• to analyze the bioavailability of phytosterol and cholesterol in the aqueous micellar phase of an in vitro simulated digestion model
• as a standard in enzyme activity assay

Uses

Cholesterol Esterase from bovine pancreas has been used:

• in γ-oryzanol-hydrolyzing activity
• in cholesterol esterase enzyme activity for quantification of cholesterol and cholesterol esters associated in tear film and lenses samples
• to test in vitro degradation of poly(fumaroyl bioxirane) maleate (PFM) polymer designed for bone tissue engineering

General Description

Cholesterol Esterase is a serine hydrolase released from the exocrine pancreas. It belongs to the α/β hydrolase family of enzymes.

Biochem/physiol Actions

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramides. The enzyme may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis. Its molecular mass is approximately 129 kDa and the optimum pH range is 7.0-9.0. The enzyme is activated by cholic acid, glycocholic acid, BSA, Mg2+ and 0.3% (v/v) ? X-100. Ag+, Hg2+ and ionic detergents inhibit the activity of the enzyme.

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