Basic information Outline Chemical Properties Uses Reaction of binary biuret Production method Safety Supplier Related
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Biuret

Basic information Outline Chemical Properties Uses Reaction of binary biuret Production method Safety Supplier Related

Biuret Basic information

Product Name:
Biuret
Synonyms:
  • (aminocarbonyl)-ure
  • Allophamicacidamide
  • Allophanimidic acid
  • allophanimidicacid
  • Dicarbamylamine
  • Dicarbonimidic diamide
  • Isobiuret
  • L-101
CAS:
108-19-0
MF:
C2H5N3O2
MW:
103.08
EINECS:
203-559-0
Product Categories:
  • Building Blocks
  • Carbonyl Compounds
  • Chemical Synthesis
  • Organic Building Blocks
  • Ureas
  • General Protein Quantitation
  • Protein Quantitation
  • Quantitation Kits and Reagents
Mol File:
108-19-0.mol
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Biuret Chemical Properties

Melting point:
185-190 °C (dec.)(lit.)
Boiling point:
100 °C
Density 
1.01 g/mL at 20 °C
refractive index 
1.4264 (estimate)
storage temp. 
2-8°C
form 
Liquid
color 
White
Odor
Odorless
Water Solubility 
2 g/100 mL (25 ºC)
Sensitive 
Hygroscopic
Merck 
14,1309
BRN 
1703510
Stability:
Stable. Incompatible with strong oxidizing agents, strong bases. Hygroscopic.
CAS DataBase Reference
108-19-0(CAS DataBase Reference)
NIST Chemistry Reference
Imidodicarbonic diamide(108-19-0)
EPA Substance Registry System
Imidodicarbonic diamide (108-19-0)
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Safety Information

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-36/37-36
WGK Germany 
3
TSCA 
Yes
HS Code 
29241990
Hazardous Substances Data
108-19-0(Hazardous Substances Data)

MSDS

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Biuret Usage And Synthesis

Outline

Biuret is the resulting product in urea production process by deamination and condensation reaction when the temperature is higher than the melting point of urea (150-170 ℃). Formula is NH2CONHCONH2. Reaction is as follows NH2CONH2-(heating)-> HNCO + NH3, HNCO + NH2CONH2 → NH2CONHCONH2, crystallization of urea in the granulation process will generate biuret, this substance is toxic to plants, thus content of biuret in urea is required not exceed 1%. Top dressing with urea, the content of biuret should not exceed 0.5%.

Chemical Properties

It is long white sheet crystals. Melting point is 190 ℃ (decomposition), the relative density is 1.467 (-5/4 ℃). Crystals in water contain 4 molecules of water of crystallization, dehydrate at about 110 ℃. It is easily soluble in alcohol, very slightly soluble in ether.

Uses

1. Biuret is used in feed for ruminants, as feed additives as urea, like, but more safe than urea.
2. It is used as pharmaceutical intermediates, growth hormone, foaming agents, paints.
3. It is used for chemical analysis.

Reaction of binary biuret

Protein produces purple in alkaline copper sulfate solution, this is because the basic copper sulfate with proteins peptide bond (-NHCO-) formed sake complexes. On the structure of purple product, generally disagree, Xu Fu (Schiff) once separated following the reaction of compound (ⅰ) (purple crystal) from the most simple binary biuret reaction mixture, the chemical reaction equation related is as follows:

Someone studied with a spectrophotometric method, suggesting that the reaction of binary biuret produces a colored substance, which is mainly composed of a tripeptide and tetrapeptide, complexes produced by ligand of copper and tripeptide was purple, complexes produced by ligand of copper with tetrapeptide was red.
This reaction is not very sensitive, because its peptide bond with another substance that has other groups conduct the biuret reaction. Some substances without the peptide bonds (e.g., histidine) may also conduct this reaction, so its work on the application is limited.

Production method

Mix urea, water with disodium hydrogen phosphate, dissolve by heating. It was heated to 150-160 ℃, heat preservation for 2h. The reaction was completed, the reactants were poured into water and allowed to stand overnight, crystals filtered off were crude, and recrystallize with dilute ammonia to give the finished product of biuret. In the production of urea, intermediate urine is as raw material, by high temperature thermal condensation, separation, and drying, the production is obtained.

Chemical Properties

white powder

Uses

Analytical reagent, especially for proteins.

Definition

ChEBI: A member of the class of condensed ureas that is the compound formed by the condensation of two molecules of urea; the parent compound of the biuret group of compounds. Used as a non-protein nitrogen source in ruminant feed.

Definition

A colorless crystalline organic compound made by heating UREA (carbamide). It is used in a chemical test for PROTEINS.

Agricultural Uses

Biuret is a compound formed from urea when the temperature during the manufacture of urea goes above 140 to 170°C. A biuret concentration of more than 2% in urea is harmful to plants as it affects the metabolism of proteins.

Purification Methods

Crystallise biuret from EtOH. [Beilstein 3 IV 141.]

Biuret Preparation Products And Raw materials

Preparation Products

Raw materials

BiuretSupplier

TOKYO CHEMICAL INDUSTRY CO., LTD. Gold
Tel
03-3668-0489
Email
Sales-JP@TCIchemicals.com
Products Intro
Product Name:Biuret [for Fertilizer Analysis]
CAS:108-19-0
Purity:>99.0% Package:1g Remarks:[for Fertilizer Analysis]
J & K SCIENTIFIC LTD.
Tel
010-82848833- ;010-82848833-
Email
jkinfo@jkchemical.com;market6@jkchemical.com
Products Intro
Product Name:Biuret, 97%
CAS:108-19-0
Purity:97% Package:100G;25G;500G;5G
Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
21-61259100-
Email
sh@meryer.com
Products Intro
Product Name:Biuret
CAS:108-19-0
Purity:99% Package:500g;1kg Remarks:B65009
future industrial shanghai co., ltd
Tel
400-0066-400;021-60496031
Email
sales@jonln.com;sales@jonln.com
Products Intro
Product Name:Biuret
CAS:108-19-0
Purity:AR,98% Package:272RMB/ 100g
Alfa Aesar
Tel
400-610-6006; 021-67582000
Email
saleschina@alfa-asia.com
Products Intro
Product Name:Biuret, 97%
CAS:108-19-0
Package:25g Remarks:L00812