N-TERT-BUTYL-ALPHA-PHENYLNITRONE Chemical Properties

Melting point:

73-74 °C(lit.)

Boiling point:

283℃

Density 

0.990

refractive index 

1.5480 (estimate)

Flash point:

119℃

storage temp. 

Store at <= 20°C.

solubility 

DMSO: soluble

form 

powder

pka

1.50±0.53(Predicted)

color 

white

Water Solubility 

Soluble in DMSO (10 mg/ml), chloroform (50 mg/ml), and water (20 mg/ml).

Merck 

14,7056

BRN 

2044028

CAS DataBase Reference

3376-24-7(CAS DataBase Reference)

EPA Substance Registry System

2-Propanamine, 2-methyl-N-(phenylmethylene)-, N-oxide (3376-24-7)

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

RTECS 

TX1760000

F 

10

TSCA 

Yes

HS Code 

2929 90 00

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

N-TERT-BUTYL-ALPHA-PHENYLNITRONE Usage And Synthesis

Chemical Properties

white to light beige fine crystalline powder

Uses

N-tert-Butyl-a-phenylnitrone is a commonly-used free radical trap. It contains radical scavenging activity and an ability to inhibit Cox-2 (cyclooxygenase-2). N-tert-Butyl-a-phenylnitrone is an antioxidant that has been shown to act as a protective agent in several experimental models of neurodegenerative disorders. N-tert-Butyl-a-phenylnitrone inhibits lipid peroxidation in rat liver microsomes. It also prevents the induction of inducible nitric oxide synthase (iNOS) by reactive oxygen species.

Uses

N-tert-Butyl-α-phenylnitrone has been used:

• as a component of Dneasy Blood&Tissue buffer to preserve the oxidized state of DNA extracted from human non-tumorigenic epithelial breast (MCF10A) cells
• as a free radical scavenger of reactive oxygen species (ROS) in microglial (MG) cell lines
• to attenuate fibroblast senescence in unstable oral squamous cell carcinomas (GU-OSCC)

Uses

Spin trap reagent for carbon- or oxygen- or nitrogen-centered free radicals.

Synthesis Reference(s)

Tetrahedron, 48, p. 8677, 1992 DOI: 10.1016/S0040-4020(01)89443-6

General Description

Most commonly-used free radical trap. An antioxidant that has been shown to act as a protective agent in several experimental models of neurodegenerative disorders. Inhibits lipid peroxidation in rat liver microsomes. Also prevents the induction of inducible nitric oxide synthase (iNOS) by reactive oxygen species.

Biochem/physiol Actions

Product does not compete with ATP.

Purification Methods

Crystallise PBN from hexane. It is a free radical trap. [cf Janzen Methods Enzymology 105 188 1984, Beilstein 7 IV 519.]

N-TERT-BUTYL-ALPHA-PHENYLNITRONE(3376-24-7)Related Product Information

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