DATP (100MM) Usage And Synthesis

Features

Chemically synthesized, pH 7.5;>99% purity confirmed by HPLC;Stable for 2 years at –20°C;Free from qPCR, PCR, reverse transcription inhibitors;Free of DNases and RNases;Free of human and E. coli DNA

Characteristic

dATP is a 100 mM clear colorless solution used in cells for synthesis of DNA by DNA polymerases. This solution is used in DNA synthesis reactions such as PCR, DNA sequencing and molecular cloning techniques. It is suitable for many applications where high-quality reagents are required. Such procedures include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.

Description

dATP is prepared to a final concentration of 100 mM in highly purified water. 100 mM dATP is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

Application

qPCR, RT-qPCR;PCR, RT-PCR, cDNA synthesis;High-fidelity and long-range PCR;Isothermal amplification;DNA labeling;Cloning;Sanger and next-generation sequencing (NGS) sequencing

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