4-Methylumbelliferyl-beta-D-glucopyranoside Chemical Properties

Melting point:

258-263 °C (dec.)

Boiling point:

394.46°C (rough estimate)

Density 

1.522

refractive index 

1.4430 (estimate)

storage temp. 

Sealed in dry,Store in freezer, under -20°C

solubility 

DMF: 5 mg/ml; DMSO: 50 mg/ml

pka

12.73±0.70(Predicted)

form 

Powder

color 

White to off-white

Water Solubility 

Soluble in DMF or water

λmax

320nm(Buffer)(lit.)

BRN 

94672

CAS DataBase Reference

18997-57-4(CAS DataBase Reference)

Safety Information

Hazard Codes 

T+

Risk Statements 

26/27/28

Safety Statements 

22-24/25

WGK Germany 

3

F 

3-10-21

HS Code 

29389090

MSDS

• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

4-Methylumbelliferyl-beta-D-glucopyranoside Usage And Synthesis

Description

4-Methylumbelliferyl-β-D-Glucopyranoside (4-MUG) is a fluorogenic substrate of β-glucosidase and β-glucocerebrosidase (also known as glucosylceramidase). In addition to its use in characterizing novel β-glucosidases, 4-MUG is used in assays to evaluate deficiency in β- glucocerebrosidase activity related to Gaucher disease. Hydrolysis of 4-MUG releases the fluorescent product 4-MU, which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.

Chemical Properties

White Powder

Uses

A fluorogenic substrate in the assay of ?-glucosidase. Fluorescence: max. Abs. 316nm; max. Em. 372nm; e x 10-3: 15

Uses

A fluorogenic substrate in the assay of ?-glucosidase.Fluorescence: max. Abs. 316nm; max. Em. 372nm; e x 10-3: 15

Uses

4-Methylumbelliferyl β-D-glucopyranoside has been used as substrate:

• in glucosylceramidase β enzyme activity assay in lysosome-enriched fractions from primary hippocampal neurons
• in β-glucosidase assay during yeast fermentation
• to assay glucocerebrosidase 1 (GBA1)-related glucosidase activity in macrophage cell line (RAW)

Definition

ChEBI: A beta-D-glucoside having a 4-methylumbelliferyl substituent at the anomeric position.

Biochem/physiol Actions

4-Methylumbelliferyl β-D-glucopyranoside is a synthetic enzymatic substrate for glycosidase. It has been used as substrate for the β- glucosidase from enterococci.

Purification Methods

4-Methylumbellifer-7-yl--Dglucopyranoside crystallises as the half hydrate from hot H2O. [Constantzas & Kocourek Collect Czech Chem Commun 24 1099 1959, De Re et al. Ann Chim (Rome) 4 9 2089 1959, Courtin-Duchateau & Veyrière Carbohydr Research 65 23, 29 1978, Beilstein 18 III/IV 5152, 8 IV 433, 18/7 V 616.]

4-Methylumbelliferyl-beta-D-glucopyranoside(18997-57-4)Related Product Information

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