Coenzyme A Chemical Properties

Density 

1.96±0.1 g/cm3(Predicted)

storage temp. 

Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

solubility 

PBS (pH 7.2): 10 mg/ml

form 

A crystalline solid

pka

9.6 (thiol); pK 6.4 (secondary phosphate); pK 4.0 (adenine NH3+)

Merck 

13,2491

BRN 

77809

LogP

-4.358 (est)

EPA Substance Registry System

Coenzyme A (85-61-0)

Safety Information

Safety Statements 

24/25

WGK Germany 

3

F 

1-10

TSCA 

Yes

HS Code 

2933299090

MSDS

• Language:English Provider:CoA
• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS

Coenzyme A Usage And Synthesis

Description

Coenzyme A (CoA) is an essential cofactor functioning as an acyl group carrier and carbonyl-activating group for the citric acid cycle and fatty acid metabolism. About 4% of cellular enzymes utilize CoA as a substrate. It is synthesized from pantothenic acid in a 5-step process that requires ATP. The pantothenate kinase step of the CoA biosynthetic pathway has been identified as a target for the development of antibacterial compounds.

Chemical Properties

yellowish lyophilisate

Uses

A hydrated form of Coenzyme A (CoA) is a useful biochemical research chemical, used in the preparation of high-yielding cell-free protein synthesis platforms.

Uses

Coenzyme A hydrate has been used in the thiolase enzyme assay of recombinant acetoacetyl-CoA thiolase (rACAT) in Clonorchis sinensis. It may be used as a reference standard in Raman spectra measurements.

Definition

ChEBI: Tetraanion of coenzyme A.

General Description

Coenzyme A is structurally composed of 2-aminoethanethiol, pantothenic acid and phosphorylated adenosine phosphate.

Biochem/physiol Actions

Coenzyme A is a cofactor involved in acyl group transfer. It is essential for carbohydrate, lipid and protein metabolism. Coenzyme A acts as a cofactor for vitamin B coenzymes.

Purification Methods

The white powder is best stored in an inert atmosphere in the dark in sealed ampoules after drying in vacuo over P2O5 at 34o. It has UV: max 259 nm ( 16,800) in H2O. [Buyske et al. J Am Chem Soc 76 3575 1954.] It is soluble in H2O but insoluble in EtOH, Et2O and M2CO. It is readily oxidised in air and is best kept as the more stable trilithium salt [Moffat & Khorana J Am Chem Soc 83 663 1961; see also Beinert et al. J Biol Chem 200 384 1953, De Vries et al. J Am Chem Soc 72 4838 1950, Gregory et al. J Am Chem Soc 74 854 1952 and Baddiley Adv Enzymol 16 1 1955]. [Beilstein 26 III/IV 3663.]

Coenzyme A(85-61-0)Related Product Information

• Coenzyme Q10
• SUCCINYL COENZYME A SODIUM SALT
• COENZYME
• COENZYME A TRILITHIUM SALT
• ACETYL COENZYME A (C2:0) LITHIUM
• Acetyl coenzyme A sodium salt
• Cocarboxylase
• D-Biotin
• NADH, disodium salt
• NAD+
• COENZYME Q9
• LAUROYL COENZYME A C12:0 LITHIUM SALT,N-DODECANOYL COENZYME A LITHIUM SALT
• NADP, Disodium Salt
• Nadph tetrasodium salt
• MALONYL COENZYME A TETRALITHIUM SALT
• 2,3-Dimethoxy-5-methyl-p-benzoquinone
• COENZYME A SODIUM SALT
• N-DECANOYL COENZYME A (C10:0)