Cy3 Chemical Properties

storage temp. 

under inert gas (nitrogen or Argon) at 2-8°C

solubility 

Water, DMSO, DMF

InChIKey

OHOQEZWSNFNUSY-UHFFFAOYSA-N

SMILES

[N+]1(CCCCCC(ON2C(=O)CCC2=O)=O)C2=C(C=C(S([O-])(=O)=O)C=C2)C(C)(C)C=1C=CC=C1C(C)(C)C2=C(N1CCCCCC(ON1C(=O)CCC1=O)=O)C=CC(S(O)(=O)=O)=C2

Cy3 Usage And Synthesis

Description

Cy3 Acid (also sold under Sulfo-Cyanine3 Acid) is a bright, water-soluble, and pH insensitive> from pH 4 to pH 10 orange-fluorescent dye. Cy3 conjugates of antibodies, peptides, and proteins can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.The carboxylic acid of Cy3 dye is a reagent of choice for the preparation of custom activated esters that often are not commercially available. Examples of such activated esters include sulfo-NHS, TFP (2,3,5,6-Tetrafluorophenol), STP (4-Sulfo-2,3,5,6-Tetrafluorophenol, Sodium Salt). Another common application for non-activated carboxylic acid is peptide modification during solid phase synthesis, which usually requires in-situ activation with peptide coupling regents, for example HATU. Cy3 Acid is also often used for control experiments, and for calibration.

Uses

CY3 is an amine reactive water soluble fluorescent dye.

Definition

ChEBI: Cy3-bifunctional dye zwitterion is a Cy3 dye and an iminium betaine. It is a conjugate acid of a Cy3-bifunctional dye(1-).

Chemical Reactivity

Non-activated

Abs/Em Maxima

555/572 nm

Extinction Coefficient

150,000 cm-1M-1

Spectrally Similar Dyes

Cy3, DyLight® 555, Alexa Fluor® 555

Cy3(146397-20-8)Related Product Information

• Cy5
• Cy7
• Cy5 DBCO
• Cy5
• diSulfo-Cy5 azide
• Sulfo-CY5.5 DBCO
• Sulfo Cy5 Amine
• sulfo-Cyanine3.5 NHS ester