Coelenterazine Chemical Properties

Melting point:

176–181 ℃ (decompose)

Boiling point:

641.4±65.0 °C(Predicted)

Density 

1.32±0.1 g/cm3(Predicted)

storage temp. 

-20°C

solubility 

methanol and ethanol: soluble

pka

9.91±0.15(Predicted)

form 

solid

color 

yellow

Sensitive 

Moisture & Light Sensitive

λmax

429nm

Biological Applications

Calciumindicator; detecting luciferase,protease,gene expression,nucleic acids,proteins,stem cells,quantum dot conjugates,superoxide; tracing protein dynamics; as a substrate for luciferase,alka

InChIKey

YHIPILPTUVMWQT-UHFFFAOYSA-N

CAS DataBase Reference

55779-48-1(CAS DataBase Reference)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36-24/25

WGK Germany 

3

F 

10

HS Code 

29339900

MSDS

• Language:English Provider:SigmaAldrich

Coelenterazine Usage And Synthesis

Chemical Properties

Yellow Solid

Uses

Coelenterazine, native was used:-

• For Aequorin assays
• For measuring the intracellular calcium concentration in response to ionomycin or PMA using aequorin in COS-7 cells
• In Bioluminescence imaging for analysing the presence of virus-dependent luciferase activity

Uses

Coelenterazine is a luminophore of the aequorin complex which is oxidized by oxygen to illuminate at 465nm when Ca2+ binds to the complex. Used to measure Ca2+ concentration in cells with high sensitivity an dlarge dynamic range.

Definition

ChEBI: An imidazopyrazine that is imidazo[1,2-a]pyrazin-3(7H)-one in which positions 2, 6, and 8 are substituted by 4-hydroxybenzyl, 4-hydroxyphenyl, and benzyl groups, respectively.

General Description

Coelenterazine is a luciferin molecule, which is utilized by several luciferases, such as Renilla,?Oplophorus and?Periphylla.

Biochem/physiol Actions

Luminophore of the aequorin complex which is oxidized by oxygen to illuminate at 465 nm when Ca2+ binds to the complex; used to measure Ca2+ concentration in cells with high sensitivity and large dynamic range.

Coelenterazine(55779-48-1)Related Product Information

• Thiophanate-methyl
• Methylparaben
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• Benzyl alcohol
• Benzyltrimethylammonium chloride
• Methyl acetate
• Methyl acrylate
• Kresoxim-methyl
• Benzyltriethylammonium chloride
• Bensulfuron methyl
• METHYL THIOPHENE-2-CARBOXYLATE
• COELENTERAZINE HCP
• COELENTERAZINE H
• Methyl
• Benzyl
• Phenylacetone
• Parathion-methyl
• Methyl bromide