3,5-Dinitrosalicylic acid Chemical Properties

Melting point:

168-172 °C (lit.)

Boiling point:

369.91°C (rough estimate)

Density 

1.7914 (rough estimate)

refractive index 

1.5300 (estimate)

storage temp. 

room temp

solubility 

H2O: soluble50mg/mL

pka

1.57±0.10(Predicted)

form 

Powder

color 

Yellow

PH Range

1.3-1.8 (10g/L @ 20°C)

Water Solubility 

soluble

BRN 

2220661

Stability:

Stable. Incompatible with strong oxidizing agents, strong bases, strong acids.

LogP

0.12 at 23℃ and pH2.6-2.8

CAS DataBase Reference

609-99-4(CAS DataBase Reference)

NIST Chemistry Reference

3,5-Dinitrosalicylic acid(609-99-4)

EPA Substance Registry System

Benzoic acid, 2-hydroxy-3,5-dinitro- (609-99-4)

Safety Information

Hazard Codes 

Xn,Xi

Risk Statements 

22-37/38-36/37/38

Safety Statements 

22-24/25-36-26

RIDADR 

2811

WGK Germany 

3

RTECS 

VO2965000

Hazard Note 

Irritant

TSCA 

Yes

HazardClass 

6.1(b)

PackingGroup 

III

HS Code 

29182990

MSDS

• Language:English Provider:3,5-Dinitrosalicylic acid
• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
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3,5-Dinitrosalicylic acid Usage And Synthesis

Chemical Properties

solid

Uses

3,5-Dinitrosalicylic acid is used in the quantification of reducing sugars by colorimetry. It is utilized for the analysis of glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. It is also used to detect reducing substances in urine and for quantifying carbohydrate level in blood. It acts as an important raw material and an intermediate in the production of organic chemicals such as agrochemicals, organic synthesis, pharmaceuticals and dyes.

Uses

3,5-Dinitrosalicylic acid was used as a reagent for the preparation of oxazolines from amino alcohols and for the spectrophotometric determination of ampicillin. It was also used to measure the effects of silver nanoparticles on the membrane leakage of the reducing sugars.

Uses

3,5-Dinitrosalicylic acid is a salicylic acid derivative used in colorimeteric determination of reducing sugars.

Definition

ChEBI: A monohydroxybenzoic acid consisting of 2-hydroxybenzoic acid having nitro substituents at the 3- and 5-positions. It is used in colorimetric testing for the presence of free carbonyl groups (C2O) in reducing sugars.

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.

Purification Methods

Crystallise the acid from H2O. [Beilstein 10 IV 270.]

3,5-Dinitrosalicylic acid(609-99-4)Related Product Information

• 4-Methylsalicylic acid
• Folic acid
• 3-Nitrosalicylic acid
• Citric acid
• Ethyl 2-(Chlorosulfonyl)acetate
• 1,3-DINITRONAPHTHALENE
• 5-Nitrosalicylic acid
• Ascoric Acid
• Methyl 3,5-dinitrosalicylate
• 3,5-DINITROSALICYLIC ACID SODIUM,SODIUM 3,5-DINITROSALICYLIC ACID,3,5-DINITROSALICYLIC ACID, MONOSODIUM SALT
• 3,5-Dinitrosalicylic acid 2-(5-nitrofurfurylidene) hydrazide,3,5-Dinitrosalicylic acid N'-(5-nitrofurfurylidene) hydrazide
• 2,4-Dinitrophenylhydrazine
• 2,5-DINITROPHENOL
• 1,3-Dinitrobenzene
• 3,5-Dinitrobenzoic acid
• Salicylic acid
• 3,5-Dinitrosalicylic acid
• 3,5-DINITROSALICYLIC HYDRAZIDE