MHY1485
MHY1485 Basic information
- Product Name:
- MHY1485
- Synonyms:
-
- MHY1485
- 4,6-dimorpholino-N-(4-nitrophenyl)-1,3,5-triazin-2-amine
- 4,6-Di-4-morpholinyl-N-(4-nitrophenyl)-1,3,5-triazin-2-amine
- MHY-1485;MHY 1485
- 1,3,5-Triazin-2-amine, 4,6-di-4-morpholinyl-N-(4-nitrophenyl)-
- 4,6-Bis(morpholin-4-yl)-N-(4-nitrophenyl)-1,3,5-triazin-2-amine
- MHY1485 USP/EP/BP
- MHY1485, 10 mM in DMSO
- CAS:
- 326914-06-1
- MF:
- C17H21N7O4
- MW:
- 387.39
- Product Categories:
-
- Inhibitors
- Mol File:
- 326914-06-1.mol
MHY1485 Chemical Properties
- Melting point:
- 256-259 °C
- Boiling point:
- 643.3±65.0 °C(Predicted)
- Density
- 1.415±0.06 g/cm3(Predicted)
- storage temp.
- 2-8°C
- solubility
- DMSO: soluble2mg/mL, clear (warmed)
- pka
- 6.19±0.10(Predicted)
- form
- powder
- color
- white to beige
- Stability:
- Stable for 1 year from date of purchase as supplied. Solutions in DMSO or DMF may be stored at -20°C for up to 3 months.
- InChI
- InChI=1S/C17H21N7O4/c25-24(26)14-3-1-13(2-4-14)18-15-19-16(22-5-9-27-10-6-22)21-17(20-15)23-7-11-28-12-8-23/h1-4H,5-12H2,(H,18,19,20,21)
- InChIKey
- MSSXBKQZZINCRI-UHFFFAOYSA-N
- SMILES
- N1=C(N2CCOCC2)N=C(N2CCOCC2)N=C1NC1=CC=C([N+]([O-])=O)C=C1
MHY1485 Usage And Synthesis
Description
MHY1485 is a cell-permeable activator of mTOR that has been shown to increase levels of cellular mTOR Ser2448 and downstream substrate 4E-BP Thr37/46 phosphorylation in rat liver Ac2F cells. It can induce cellular LC3-II accumulation in Ac2F cells and has been shown to inhibit autophagy by suppressing the fusion between autophagosomes and lysosomes.
Uses
MHY1485 has been used:
- to study the effect of mammalian target of rapamycin mTOR signalling on in vitro O-GlcNAcylation
- to inhibit autophagy
- as a mTOR agonist to demonstrate that the O-linked N-acetylglucosamine transferase- RNA helicase p68 (OGT-DDX5) axis regulates colorectal cancer cell proliferation and metastasis
Biochem/physiol Actions
MHY1485 is mTOR activator that potently inhibits autophagy by suppression of fusion between autophagosomes and lysosomes.
in vivo
MHY1485 (intraperitoneal injection; 10?mg/kg, 2 days) blocks the autophagy signaling induced by follicle-stimulating hormone (FSH). It increases p-mTOR and p-S6K1 expression levels, whereas LC3 expression shows no marked change compared to that in the control group[3].
| Animal Model: | 4-week-old female ICR mice[3] |
| Dosage: | 10?mg/kg, 2 days |
| Administration: | Intraperitoneal injection |
| Result: | Suppressed the autophagy level following FSH treatment.? |
IC 50
mTORC1; mTORC2; Autophagy
References
[1] YEON JA CHOI. Inhibitory effect of mTOR activator MHY1485 on autophagy: suppression of lysosomal fusion.[J]. PLoS ONE, 2012: e43418. DOI:10.1371/journal.pone.0043418
[2] YUAN CHENG. Promotion of ovarian follicle growth following mTOR activation: synergistic effects of AKT stimulators.[J]. PLoS ONE, 2015: e0117769. DOI:10.1371/journal.pone.0117769
[3] SAI ZHAO . MHY1485 activates mTOR and protects osteoblasts from dexamethasone[J]. Biochemical and biophysical research communications, 2016, 481 3: Pages 212-218. DOI:10.1016/j.bbrc.2016.10.104
[4] BO YANG. MHY1485 ameliorates UV-induced skin cell damages via activating mTOR-Nrf2 signaling[J]. Oncotarget, 2017, 8 1: 12775-12783. DOI:10.18632/oncotarget.14299
[5] CAIXIA LI Helmy M S. (Pro)renin receptor regulates autophagy and apoptosis in podocytes exposed to high glucose.[J]. American journal of physiology. Endocrinology and metabolism, 2015, 309 3: E302-10. DOI:10.1152/ajpendo.00603.2014
MHY1485Supplier
- Tel
- sales@boylechem.com
- Tel
- +86-21-20908456
- sales@BioChemBest.com
- Tel
- 400-6009262 16621234537
- chenyj@titansci.com
- Tel
- 0411-62910999 13889544652
- sales@meilune.com
- Tel
- 025-66113011 17798518460
- cfzhang@aikonchem.com