Azido-PEG3-Maleimide
Azido-PEG3-Maleimide Basic information
- Product Name:
- Azido-PEG3-Maleimide
- Synonyms:
-
- Azido-PEG3-Maleimide
- N3-PEG3-Mal
- Azide-PEG3-maleimide
- Azido-PEG4-BMPA
- 1H-Pyrrole-1-propanamide, N-[2-[2-[2-(2-azidoethoxy) ethoxy] ethoxy] ethyl]-2,5-dihydro-2,5-dioxo-
- CAS:
- 1858264-36-4
- MF:
- C15H23N5O6
- MW:
- 369.37302
- Product Categories:
-
- peg
- Mol File:
- 1858264-36-4.mol
Azido-PEG3-Maleimide Usage And Synthesis
Description
Azido-PEG3-Maleimide is provided as a kit of two vials. Azido-PEG3-Maleimide is not stable for storage; it needs to be made in situ by mixing Azido-PEG3-amine and Maleimide-NHS ester followed by shaking/ stirring for 30 minutes at room temperature. The maleimide group of the Azido-PEG3-Maleimide can react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. Azide group enables Click Chemsitry reactions.
Biological Activity
Azido-PEG3-maleimide is a PROTAC linker belonging to the PEG class, which can be used to synthesize PROTAC molecules. Azido-PEG3-maleimide is also a cleavable ADC linker containing 3-unit PEG, which can be used to synthesize antibody drug conjugates (ADCs).
in vitro
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.
ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker.