Cyanine3 maleimide Chemical Properties

solubility 

Soluble in DMSO, DMF, DCM

form 

Solid

color 

Light yellow to brown

Appearance

red powder

InChIKey

WONAOVDQEMKIMH-UHFFFAOYSA-N

SMILES

C(N1C(C(C)(C)C2=CC=CC=C12)=CC=CC1C(C2=CC=CC=C2[N+]=1C)(C)C)CCCCC(=O)NCCN1C(C=CC1=O)=O.[Cl-]

Cyanine3 maleimide Usage And Synthesis

Description

Cy3 maleimide is a mono-reactive dye containing maleimide group, which can selectively and efficiently attach Cyanine3 fluorophore(an analog of Cy3?) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides). Sulfo-Cy3 maleimide version is available for the labeling of antibodies and other sensitive proteins.

Uses

Cy3 maleimide chloride is a dye derivative of Cyanine 3 (Cy3) (HY-D0822) containing maleimide functional groups. Cy3 is a fluorescent dye with a fluorescence spectrum typically in the green to orange wavelength range. The alkyne functional group of Cy3 maleimide chloride can undergo a "thiol-acrylamide" reaction with molecules containing sulfur-oxygen functional groups to form covalent bonds. Cy3 maleimide chloride can bind to biological molecules such as proteins and antibodies to track their location and dynamic changes in biological samples.

Abs/Em Maxima

555/570 nm

Fluoresene quantum yield

0.31

Extinction Coefficient

150000

CF260

0.04

CF280

0.09