6-CARBOXYFLUORESCEIN N-SUCCINIMIDYL ESTER Chemical Properties

Melting point:

>168°C (dec.)

Boiling point:

788.1±70.0 °C(Predicted)

Density 

1.74±0.1 g/cm3(Predicted)

storage temp. 

−20°C

solubility 

Soluble in N,N-dimethylformamide,dimethyl sulfoxide

pka

9.31 ± 0.20, most acidic, temperature:25 °C

form 

Solid

color 

Orange solid

λmax

496 nm (Buffer pH9)

Stability:

Moisture Sensitive

Biological Applications

Analyzing aminoglycosides; analyzing/determining gabapentin; detecting/analyzing baclofen in human plasma at micromolar level; determining phosphorus-containing amino acid herbicides; multiplex genetic analysis; visualizing/detecting proteins; for synthesizing vancomycin analogs, vasopressin analogs; as a substrate for measuring kinase activity, phosphatase activity.

CAS DataBase Reference

92557-81-8(CAS DataBase Reference)

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

F 

8-10-21

HS Code 

29252900

MSDS

• Language:English Provider:SigmaAldrich

6-CARBOXYFLUORESCEIN N-SUCCINIMIDYL ESTER Usage And Synthesis

Chemical Properties

Yellow to orange powder

Uses

(6-FAM-SE) 6-Carboxyfluorescein-N-hydroxysuccinimide Ester is a commonly used fluorescent reagent for the labelling of peptides, proteins and nucleotides.

Uses

Intracellular pH probe. Fluorescent label for amines and alcohols.

Definition

ChEBI: 6-carboxyfluorescein succinimidyl ester is an N-hydroxysuccinimide ester derived from 6-carboxyfluorescein. A fluorescent cell staining dye, it is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably to intracellular lysine residues. It has a role as a fluorochrome. It is functionally related to a fluorescein (lactone form).

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