Girard's Reagent T Chemical Properties

Melting point:

188-192 °C (dec.) (lit.)

Density 

1.3799 (rough estimate)

refractive index 

1.5400 (estimate)

storage temp. 

Inert atmosphere,Room Temperature

form 

powder to crystal

color 

White to Almost white

Water Solubility 

soluble

Merck 

14,4429

BRN 

3727163

InChI

InChI=1S/C5H13N3O.ClH/c1-8(2,3)4-5(9)7-6;/h4,6H2,1-3H3;1H

InChIKey

YSULOORXQBDPCU-UHFFFAOYSA-N

SMILES

[N+](C)(C)(C)CC(=O)NN.[Cl-]

CAS DataBase Reference

123-46-6(CAS DataBase Reference)

EPA Substance Registry System

Ethanaminium, 2-hydrazino-N,N,N-trimethyl-2-oxo-, chloride (123-46-6)

Safety Information

Hazard Codes 

Xn-F,Xn,F

Risk Statements 

44-36/37/38-20/22-11-R44-R36/37/38-R20/22-R11

Safety Statements 

22-24/25-36/37/39-26-16-S36/37/39-S26-S16

WGK Germany 

3

RTECS 

CP3150000

F 

21

TSCA 

TSCA listed

HS Code 

29280000

Storage Class

11 - Combustible Solids

MSDS

• Language:English Provider:2-Hydrazino-N,N,N-trimethyl-2-oxo-ethanaminium chloride
• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS

Girard's Reagent T Usage And Synthesis

Chemical Properties

white to almost white crystalline powder

Uses

Girard''s Reagent T is a derivatizing agent used to generate permanent cationic charge at the reducing ends of neutral oligosaccharides for easier quantification in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).

Uses

Girard′s reagent T may be used as a derivatizing reagent for the determination of 5-Formyl-2′-deoxyuridine in cellular DNA samples using liquid chromatography–tandem mass spectrometry (LC–MS/MS) technique. Reducing saccharides can be derivatized by Girard′s reagent T and this can be analyzed by matrix-assisted laser desorption/ionization coupled to time of flight mass spectrometry (MALDI-TOF-MS) technique.

Purification Methods

It is purified by crystallisation from absolute EtOH (slight decomposition) until it has only a slight odour. Store it in well-stoppered containers because it is very hygroscopic. It is very soluble in H2O, AcOH and glycerol but slightly soluble in EtOH (0.66%). It forms water-soluble hydrazones with carbonyl compounds. [Beilstein 4 III 1133.]

Girard's Reagent T(123-46-6)Related Product Information

• N-ACETYL-L-TYROSINE ETHYL ESTER
• Trimethyl borate
• Adipic dihydrazide
• Calcium chloride
• Benzyltriethylammonium chloride
• (Chloromethylene)dimethyliminium chloride
• Trimethylamine hydrochloride
• Tetramethylammonium chloride
• Sodium chloride
• Bethanechol
• Tebufenozide
• Choline chloride
• Sulfuryl chloride
• Ammonium chloride
• Thionyl chloride
• BLOCKING REAGENT GE
• Polyvinyl chloride
• Potassium chloride