IONOMYCIN CALCIUM SALT Chemical Properties

Melting point:

205-206 C

Flash point:

87 °C

storage temp. 

−20°C

solubility 

Soluble in DMSO (10mg/ml). Also soluble in ethanol or methanol

form 

White to off-white solid

color 

white to off-white

biological source

Streptomyces conglobatus

Sensitive 

Light Sensitive

BRN 

1416163

Stability:

Stable for 1 year from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 2 months.

Safety Information

Hazard Codes 

Xn

Risk Statements 

22

Safety Statements 

36

WGK Germany 

3

RTECS 

NO0650000

HazardClass 

3

HS Code 

29419090

MSDS

• Language:English Provider:SigmaAldrich

IONOMYCIN CALCIUM SALT Usage And Synthesis

Description

Ionomycin is a calcium ionophore originally isolated from S. conglobatus. It induces intracellular calcium mobilization in X. laevis oocytes when used at a concentration of 5 μM. Ionomycin (5 μM) induces the formation of neutrophil extracellular traps (NETs) in isolated human neutrophils. Ionomycin in combination with phorbol 12-myristate 13-acetate (PMA; ) has commonly been used to induce the proliferation and activation of T cells, as well as to stimulate cytokine production in macrophages, in vitro.

Chemical Properties

Powder

Uses

Ionomycin is more effectivethan A23187 as a Ca++ionophore; Ionomycin is used in research on Ca++ transport across biological membranes; Ionomycin induces apoptotic degeneration of embryonic cortical neurons.

Uses

Ionomycin calcium salt is used as a polyether antibiotic and apoptotic degeneration inducer. Ionomycin induces apoptotic degeneration of embryonic cortical neurons and cell cycle arrest at G1 phase. It is more effective than A23187 (sc-3591) and provides a useful tool for increasing intracellular Ca2+ concentration.

General Description

Useful in cell activation experiments when calcium dose-response data are not required. Also available as a 10 mM solution in DMSO (Cat. No. 407953).

Biological Activity

Calcium ionophore; more specific than A23187 (5-(Methylamino)-2-[[2R,3R,6S,8S,9R,11R)-3,9,11-trimethyl-8-[(1S)-1-methyl-2-oxo-2-(1H-pyrrol-2-yl)-ethyl]-1,7-dioxaspiro[5.5]undec-2-yl]methyl]-4-benzoxazolecarboxylic acid ).

Biochem/physiol Actions

Product does not compete with ATP.

storage

-20°C

References

Liu et al. (1978), Ionomycin, a new polyether antibiotic; J. Antibiot., 31 815 Liu and Hermann et al. (1978), Characterization of ionomycin as a calcium ionophore; J. Biol.Chem, 253 5892

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