EOSIN Chemical Properties

Melting point:

>300°C

Density 

1.02 g/mL at 20 °C

vapor pressure 

0Pa at 25℃

Flash point:

11 °C

storage temp. 

Store at RT.

solubility 

H₂O: 0.1 g/mL, dark red

Colour Index 

45380

pka

2.9, 4.5(at 25℃)

form 

Solid

color 

Reddish

PH

9.2 (10g/l, H2O, 20℃)

PH Range

0(yellow)-3(green fluoresc.)

Water Solubility 

freely soluble

λmax

514nm

Merck 

14,3603

BRN 

3586809

Stability:

Stable. Incompatible with strong oxidizing agents.

Biological Applications

Treating age-related macular degeneration,burns,cancer,diabetes,obesity,dental bone defects,gastroesophageal reflux disease,prostate cancer,viral diseases; stents; wound-healing materials

Major Application

Photovoltaic devices, color filter, nanoscale host material, detection of latent fingerprints, inks, detergents, hair dyes, cosmetics, implantable drug delivery devices, stents, diagnosis of breast lesions, labeling nucleic acids, detecting proteins, stress biomarkers, phosphoprotein, breast cancer metastases, antimicrobial agent, treatment of burns, endodontic, diabetes, obesity, cancer, radiochemotherapy, DNA sequencing, gene expression profiling, wound healing promoters

LogP

-1.33

CAS DataBase Reference

17372-87-1(CAS DataBase Reference)

EPA Substance Registry System

Eosin Yellowish (YS) (17372-87-1)

Safety Information

Hazard Codes 

F,T,Xn,Xi

Risk Statements 

11-23/24/25-39/23/24/25-52/53-41-22-36-10

Safety Statements 

7-16-36/37-45-24/25-61-39-26

RIDADR 

UN 1230 3/PG 2

WGK Germany 

1

RTECS 

LM5850000

TSCA 

Yes

HS Code 

32041200

Toxicity

LD50 orally in Rabbit: > 2000 mg/kg

MSDS

• Language:English Provider:C.I. 45380
• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich

EOSIN Usage And Synthesis

Chemical Properties

brownish-red powder

Uses

Eosin is most often used as a counterstain to haematoxylin in H&E (haematoxylin and eosin) staining. H&E staining is one of the most commonly used techniques in histology.

Uses

Anti Septic

Uses

Eosin Yellowish noted to reduce the affinity of Mg2+-ATPase for ATP and increase the enzyme affinity for Mg2+ at concentrations of 10-50 ?M. At higher concentrations near 100 ?M the affinity for Mg2+-ATPase for the ion-activator has been shown to be reduced. This compound has also been reported to be a photosensitizer that works as a molecular photoelectrode via catalyzation of the visible-light-driven electron-transfer reaction. Eosin Y is also used to stain structures such as muscle, cytoplasm, and collagen. This agent also enhances fluorescent quenching abilities by forming a complex with polyvinulpyrrolidone.

Preparation

countries called red. Fluorescent element (C.I. Acid Yellow 73, C.I. 45350) in water or ethanol solution bromine into four bromine derivatives, and then into sodium salt.

Definition

ChEBI: An organic sodium salt that is 2',4',5',7'-tetrabromofluorescein in which the carboxy group and the phenolic hydroxy group have been deprotonated and the resulting charge is neutralised by two sodium ions.

Flammability and Explosibility

Not classified

Biological Activity

ami5/eosin y disodium salt is a non-selective protein methyltransferase inhibitor.post-translational protein methylation at lysine and arginine residues is related to the gene expression regulation. the enzymatic activities of protein methyltransferases serve to do covalent modifications in the control of gene transcription.

Safety Profile

Poison by intravenous and intraperitoneal routes. Moderately toxic by ingestion. Questionable carcinogen with experimental tumorigenic data. When heated to decomposition it emits very toxic fumes of Brand NazO. See also BROMIDES.

in vitro

ami5/eosin y disodium salt has been identified as a competitive inhibitor of sam binding and had been shown to inhibit not only prmts but also lysine methylation by the set7 and disruptor of telomeric silencing 1-like (dot1l) mtases in vitro. both ami5/eosin y disodium salt and its analog ami-1 have been used as lead compounds for the development of novel mtase-specific inhibitors. moreover, it was found that ami5/eosin y disodium salt could inhibit set7 in vitro and decrease h3k4m1 in vascular endothelial cells. [1].

IC 50

0.78 and 1.41 μm for hmt1p and prmt1, respectively

storage

+4°C

Purification Methods

Dissolve it in the minimum volume of H2O (1g/mL), filter and add EtOH until separation of salt is complete. Filter the solid off, wash it with absolute EtOH, then Et2O and dry it first in air, then at 100o. It is used for staining blood cells and for estimating traces of Ag. [Selsted & Becker Anal Biochem 155 270 1986, El-Ghamry & Frei Anal Chem 40 1986 1968.] [Beilstein 19 III/IV 2917.] [See above]

Properties and Applications

yellow peach. Orange powder, soluble in water and ethanol with fluorescent blue light is yellowish red solution. Meet strong sulfuric acid yellow, it will be diluted yellow light red precipitate generated. Dyeing copper ions in the blue colour and lustre; Iron ion colour and lustre in the dark blue. Discharge the gender is good. Mainly used in red ink and red pencils, only comfortable carpet dyeing. After refining can be used as medicine and cosmetics of coloring. Can also be used in leather dyeing. The aluminum salt can be used as organic pigments.

Standard	Light Fastness	Soaping		Persperation Fastness	Oxygen bleaching		Fastness to seawater
		Fading	Stain		Fading	Stain	Fading	Stain
ISO	2	3	3	2	1	2	2	2
AATCC	1	2	2	3	1	1	3	3

References

[1] okabe j,fernandez az,ziemann m,keating st,balcerczyk a,el-osta a. endothelial transcriptome in response to pharmacological methyltransferase inhibition. chemmedchem.2014 aug;9(8):1755-62.

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