2,3,5-Triphenyltetrazolium chloride Chemical Properties

Melting point:

250 °C(lit.)

Boiling point:

490.96°C (rough estimate)

Density 

1.1704 (rough estimate)

refractive index 

1.6110 (estimate)

storage temp. 

2-8°C

solubility 

H₂O: may be clear to slightly hazy

form 

powder

color 

faint yellow

Water Solubility 

Soluble in water, ethanol and acetone. Insoluble in ether.

λmax

247 nm

Sensitive 

Light Sensitive & Hygroscopic

Merck 

14,9744

BRN 

3923356

Stability:

Stable. Light sensitive. Protect from moisture. Incompatible with strong oxidizing agents.

Biological Applications

Algae viability assay; microbial growth assays; transketolase activity screening assays; detecting bacteria,g-hydroxybutyric acid (GHB),microorganisms,myocardial infarction; measuring dehydrogenase activity; treating cancer

InChIKey

PKDBCJSWQUOKDO-UHFFFAOYSA-M

CAS DataBase Reference

298-96-4(CAS DataBase Reference)

EPA Substance Registry System

2,3,5-Triphenyltetrazolium chloride (298-96-4)

Safety Information

Hazard Codes 

Xi,Xn

Risk Statements 

36/37/38-20/21/22

Safety Statements 

26-36

WGK Germany 

3

RTECS 

XF8100000

F 

3-8-10

TSCA 

Yes

HS Code 

29339900

Toxicity

LD50 intravenous in mouse: 5600ug/kg

MSDS

• Language:English Provider:2,3,5-Triphenyl-2H-tetrazolium chloride
• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

2,3,5-Triphenyltetrazolium chloride Usage And Synthesis

Chemical Properties

white powder

Uses

2,3,5-Triphenyl-2H-tetrazolium chloride is used as a dye in cell biological studies.

Uses

2,3,5-Triphenyltetrazolium chloride is a selective serotonin 5-HT4 receptor agonist and 5-HT3 gastrointestinal prokinetic agent for functional dyspepsia

Uses

In analytical chemistry as a sensitive reagent for reducing sugars, and to distinguish between a-ketols and simple aldehydes. For staining plant and animal tissue. Germination indicator in testing the ability of seeds to germinate: G. Lakon, Ber. Dtsch. Bot. Ges. 60, 299, 434 (1942). In histochemical studies. In determination of antibiotics; of dehydrogenases.

Definition

ChEBI: An organic chloride salt having 2,3,5-triphenyltetrazolium as the counterion.

General Description

2,3,5-Triphenyltetrazolium chloride is a colorless water-soluble dye. In the mitochondria of living cells, it is reduced to a deep red, water-insoluble compound (formazan). 2,3,5-Triphenyltetrazolium chloride helps to distinguish between viable and infarcted brain tissue after stroke.

Purification Methods

Crystallise TTZ from EtOH or CHCl3, and dry it at 105o. [Beilstein 26 H 363, 26 II 216, 26 III/IV 1774.]

2,3,5-Triphenyltetrazolium chloride(298-96-4)Related Product Information

• 5-Phenyltetrazole
• Ammonium chloride
• Calcium chloride
• Mepiquat chloride
• 5-Methyl-1H-tertazole
• Tetrazole
• Triphenylphosphine
• Benzyl chloride
• Sodium chloride
• Nitrotetrazolium blue chloride
• Tetrazolium Violet
• 1H-Tetrazole-1-acetic acid
• Tris(triphenylphosphine)rhodiu
• Polyvinyl chloride
• Trimethylphenylammonium chloride
• Choline chloride
• Chlorodiphenylphosphine
• Iodonitrotetrazolium chloride
• Tetrazolium Chloride