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Xylene cyanol FF

Basic information Safety Supplier Related

Xylene cyanol FF Basic information

Product Name:
Xylene cyanol FF
Synonyms:
  • 4-[[4-(Ethylamino)-3-methylphenyl][4-(ethylimino)-3-methyl-2,5-cyclohexadien-1-ylidene]methyl]-6-hydroxy-1,3-benzenedisulfonic acid sodium salt
  • 4-[(Z)-[4-(ethylamino)-3-methylphenyl]-(4-ethylimino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-2-hydroxy-5-sulfobenzenesulfonate
  • Xylene cyanol FF, pure 5GR
  • Xylene Cyanol, pure
  • Xylene Blue FF
  • Trypan Blen
  • 5-cyclohexadien-1-ylidene]methyl]-6-hydroxy--methyl-monosodiumsalt
  • CI 42135
CAS:
4463-44-9
MF:
C25H29N2NaO7S2
MW:
556.62
EINECS:
224-728-5
Mol File:
4463-44-9.mol
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Xylene cyanol FF Chemical Properties

Melting point:
295°C (dec.)
storage temp. 
2-8°C(protect from light)
form 
Powder Solid
color 
Purple
InChIKey
PFTKLWOBGKYYTB-WOEHIUECSA-N
SMILES
C(=C1C=C/C(=N/CC)/C(C)=C1)(C1C=CC(NCC)=C(C)C=1)C1C=C(O)C(S(O)(=O)=O)=CC=1S(O)(=O)=O.[NaH]
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Safety Information

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-36-37/39
WGK Germany 
3
HS Code 
32041200

MSDS

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Xylene cyanol FF Usage And Synthesis

Description

Xylene Cyanol FF is an acid triphenylmethane dye. It can be used for histochemical staining of hemoglobin peroxidase or as a tracking dye for DNA sequencing in electrophoresis.
The Xylene Cyanol FF and Bromophenol Blue are used to monitor the agarose gel while running. Xylene cyanol FF, Bromophenol blue, and Orange G are used to track DNA migration. The Xylene cyanol FF, Bromophenol blue and Orange G migrate at approximately 800 bp, 150 bp and 30 bp on a standard 2% TAE agarose gel, respectively (4,000 bp, 500 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well, and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Chemical Properties

dark red to dark blue crystalline powder

Xylene cyanol FFSupplier

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