3-Cyclohexylaminopropanesulfonic Acid Chemical Properties

Melting point:

>300 °C(lit.)

Density 

1.1809 (rough estimate)

vapor pressure 

0Pa at 25℃

refractive index 

1.5250 (estimate)

Flash point:

>110℃

storage temp. 

room temp

solubility 

H₂O: 0.5 M at 20 °C, clear

form 

Powder/Solid

color 

White

Odor

Odorless

PH Range

3 - 7

PH

3.0-7.0 (20℃, 0.5M in H2O)

pka

10.4(at 25℃)

Water Solubility 

9 g/100 mL (20 ºC)

λmax

λ: 260 nm Amax: ≤0.04
λ: 280 nm Amax: ≤0.04

Merck 

14,1767

BRN 

2835588

Stability:

Stable. Incompatible with strong oxidizing agents.

LogP

0.3 at 25℃

CAS DataBase Reference

1135-40-6(CAS DataBase Reference)

EPA Substance Registry System

1-Propanesulfonic acid, 3-(cyclohexylamino)- (1135-40-6)

Safety Information

Hazard Codes 

Xi,Xn

Risk Statements 

36/38-36/37/38-22-20/21/22-40/22

Safety Statements 

26-36/37/39-37/39-24/25-22-23-36

WGK Germany 

3

RTECS 

TZ6395000

F 

34

TSCA 

Yes

HS Code 

29213099

MSDS

• Language:English Provider:3-(Cyclohexylamino)-1-propanesulfonic acid
• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

3-Cyclohexylaminopropanesulfonic Acid Usage And Synthesis

Chemical Properties

White/clear crystalline powder

Description

3-Cyclohexylaminopropanesulfonic Acid, also known as 3-(Cyclohexylamino)-1-propanesulfonic acid (CHAPS) or N-Cyclohexyl-3-aminopropanesulfonic acid, is a detergent with a high affinity for basic proteins that can be used to extract these proteins from biological samples and solubilize them. CHAPS is a type of ampholyte, which means it has both anionic and cationic groups.

Uses

3-Cyclohexylaminopropanesulfonic Acid buffer salt is used to formulate CAPS buffer, a zwitterionic buffer that is useful in the range of pH 7.9-11.1. CAPS buffer is widely used in Western and immunoblotting experiments as well as protein sequencing and identification. Used in the electrotransfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724). The high pH of this buffer makes it useful for the transfer of proteins with a pI > 8.5. CAPS is not one of the original Good's buffers, although it has a similar structure to the other propanesulfonic acids and was selected as a highly water soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins, minimal salt effects. It is a biological buffer.

Definition

ChEBI: Cyclohexylaminopropanesulfonic acid is an amine.

Flammability and Explosibility

Not classified

References

[1] David A. Paulls, A. Townshend. “Sensitized determination of sulfite using flow injection with chemiluminescent detection.” Analyst 6 1 (1995): 467–469.
[2] Takashi Yoshitake. “Simultaneous determination of norepinephrine, serotonin, and 5-hydroxyindole-3-acetic acid in microdialysis samples from rat brain by microbore column liquid chromatography with fluorescence detection following derivatization with benzylamine.” Analytical biochemistry 312 2 (2003): 125–33.
[3] Keiichi Fujimori. “Determination of Sulfide with Acidic Permanganate Chemiluminescence for Development of Deep-sea in-situ Analyzers.” Analytical Sciences 27 2 (2011): 183–186.

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