HEPES sodium salt Chemical Properties

Melting point:

234 °C

Density 

1.504[at 20℃]

vapor pressure 

0Pa at 25℃

storage temp. 

room temp

solubility 

H₂O: 1 M at 20 °C, clear, colorless

form 

dry powder

color 

White

PH Range

6.8 - 8.2

PH

10.0-12.0 (1M in H2O)

pka

7.5(at 25℃)

biological source

synthetic

Water Solubility 

It is soluble in water.

Sensitive 

Hygroscopic

Stability:

Stable. Hygroscopic - protect from moisture. Incompatible with strong oxidizing agents.

CAS DataBase Reference

75277-39-3(CAS DataBase Reference)

EPA Substance Registry System

1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, monosodium salt (75277-39-3)

Absorption

≤0.2 at 260 in H2O at 1M
≤0.2 at 280 in H2O at 1M

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

F 

3-10

TSCA 

Yes

HS Code 

29335995

MSDS

• Language:English Provider:Sodium 4-(2-hydroxyethyl)piperazin-1-ylethanesulphonate
• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

HEPES sodium salt Usage And Synthesis

Chemical Properties

white powder

Uses

HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Definition

ChEBI: Sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate is an organic sodium salt, a N-(2-hydroxyethyl)piperazine and a N-(sulfoalkyl)piperazine. It contains a 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate.

General Description

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of 6.8 to 8.2.

Flammability and Explosibility

Not classified

Biological Activity

Multi purpose buffer used in biological research.

Synthesis

The general procedure for the synthesis of N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium salt from N-hydroxyethylpiperazine and sodium 2-hydroxyethanesulfonate is as follows: magnetic stirring in a 70-mL fluoropolymer-lined steel autoclave equipped with internal thermocouples for monitoring the temperature of the liquid phase, a pressure gauge, a pressure-reducing valve, and an exhaust valve. First, amine feedstock, anhydrous or aqueous sodium 2-hydroxyethanesulfonate solution, and anhydrous or aqueous sodium hydroxide solution were added to the open-topped autoclave. Subsequently, the autoclave was assembled and immersed in an electrically heated oil bath. The stirred reaction mixture was rapidly heated to the specified reaction temperature with the exhaust valve closed and maintained at that temperature for the time specified in Examples 9, 10 and 13. Upon completion of the reaction, the mixture was cooled to room temperature, the reactor was opened, and the reaction product was dissolved in a sufficient amount of water to ensure complete dissolution of all solids.

storage

Room temperature

References

[1] Patent: US2006/89509, 2006, A1. Location in patent: Page/Page column 4-6
[2] Patent: US2006/89509, 2006, A1. Location in patent: Page/Page column 4-6

HEPES sodium salt(75277-39-3)Related Product Information

• 2-(METHYLSULFONYL)ETHANOL
• Piperazine citrate
• Sodium carbonate
• N-Aminoethylpiperazine
• 2-Hydroxyethanesulphonic acid
• Sodium benzoate
• Calcium dobesilate
• 2-Ethylpiperazine
• Sodium chloride
• Sodium bicarbonate
• Sodium isethionate
• Sodium formate
• HEPES
• 2-Piperidinoethanol
• Sodium acetate
• Hydroxyethyl starch
• Piperazine
• Ethanesulfonic acid