Hoechst 33342 Chemical Properties

Boiling point:

725.9±70.0 °C(Predicted)

Density 

1.274±0.06 g/cm3(Predicted)

storage temp. 

2-8°C

solubility 

H2O: soluble20mg/mL

form 

Yellow solid

pka

10.97±0.10(Predicted)

Water Solubility 

Water : 1 mg/mL (2.21 mM; ultrasonic and warming and heat to 80°C)

BRN 

1234011

CAS DataBase Reference

23491-52-3(CAS DataBase Reference)

Safety Information

Hazard Codes 

Xn,C

Risk Statements 

22-37/38-36/37/38-20/21/22-37-35

Safety Statements 

22-36/37-24/25-36-26-45-36/37/39

WGK Germany 

3

RTECS 

DT4200000

F 

3-8-10

HS Code 

32129000

Toxicity

dni-hmn-hla 300 nmol/L CRNGDP 13,2389,1992

MSDS

• Language:English Provider:2'-(4'-Ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bis-1H-benzimidazole trihydrochloride trihydrate
• Language:English Provider:SigmaAldrich

Hoechst 33342 Usage And Synthesis

Chemical Properties

yellow powder

Uses

Hoechst 33342 is an A:T-specific DNA minor groove ligand that is a widely used fluorochrome for visualizing cellular DNA. Excitation of the dye occurs around 350 nm with an emission maxima around 461 nm. Hoechst-33342, unlike other Hoechst dyes, is more lipophillic due to an additional ethyl group. The compound has been observed to displace several known DNA intercalators. Adriamycin has been shown to disrupt the DNA dye update. The compound can be used in sorting living cells based on DNA content by FACS, in the visualization of chromatin distribution in living cells, to detect BrdU incorporation into cells, the visualization of fixed cells by microscopy, and to study the initial stages of apoptosis and cell-cycle distribution. In addition to Hoechst-33342''s fluorescent capability, the

Uses

Hoechst 33342 is useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry. Suitable for vital DNA staining of a variety of cell types and as a probe of membrane permeability in mammalian cells.

Definition

ChEBI: 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole is a bibenzimidazole and a N-methylpiperazine. It has a role as a fluorochrome. It is functionally related to a pibenzimol.

Safety Profile

Mutation data reported. Whenheated to decomposition it emits toxic vapors of NOx.

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