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3-aminophenoxazone

Product Name
3-aminophenoxazone
CAS No.
1916-59-2
Chemical Name
3-aminophenoxazone
Synonyms
Phx-3;Questiomycin A;2-Aminophenoxazon;3-aminophenoxazone;2-AMino-3-phenoxazone;2-amino-1H-phenoxazin-3-one;2-Amino-3-oxo-3H-phenoxazine;3H-Phenoxazin-3-one, 2-aMino-;2-AMino-3H-phenoxazin-3-one, 2-AMinophenoxazone, AV Toxin C, NSC 94945
CBNumber
CB01387919
Molecular Formula
C12H8N2O2
Formula Weight
212.2
MOL File
1916-59-2.mol
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3-aminophenoxazone Property

Melting point:
249-250 °C
Boiling point:
352.04°C (rough estimate)
Density 
1.2399 (rough estimate)
refractive index 
1.6000 (estimate)
storage temp. 
Store at -20°C
solubility 
DMF: 2mg/mL; DMSO: 3mg/mL; DMSO:PBS (pH 7.2) (1:20): 0.04mg/mL
form 
A solid
pka
2.73±0.20(Predicted)
color 
Brown to black
InChI
InChI=1S/C12H8N2O2/c13-7-5-9-12(6-10(7)15)16-11-4-2-1-3-8(11)14-9/h1-6H,13H2
InChIKey
RDJXPXHQENRCNG-UHFFFAOYSA-N
SMILES
C1C2C(OC3=C(N=2)C=CC=C3)=CC(=O)C=1N
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Cayman Chemical
Product number
27623
Product name
Questiomycin A
Packaging
1mg
Price
$68
Updated
2024/03/01
Cayman Chemical
Product number
27623
Product name
Questiomycin A
Packaging
10mg
Price
$570
Updated
2024/03/01
Cayman Chemical
Product number
27623
Product name
Questiomycin A
Packaging
5mg
Price
$319
Updated
2024/03/01
TRC
Product number
Q509800
Product name
QuestiomycinA
Packaging
10mg
Price
$500
Updated
2021/12/16
AK Scientific
Product number
2266BA
Product name
2-Amino-3H-phenoxazin-3-one
Packaging
1mg
Price
$174
Updated
2021/12/16
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3-aminophenoxazone Chemical Properties,Usage,Production

Description

Questiomycin A is a phenoxazine and a chromophore that has been found in Streptomyces and has antibacterial and anticancer activities. It is active against M. scrofulaceum, M. marinum, and M. intracellulare (MICs = 2.8, 11.3, and 5.6 μg/ml, respectively) but not M. tuberculosis, M. smegmatis, M. kansasii, or M. fortuitum (MICs = >45 μg/ml). It is also inactive against E. coli, P. aeruginosa, S. tymphimurium, S. aureus, or L. monocytogenes. It is cytotoxic to a variety of cancer cells, including MCF-7, A549, MIA PaCa-2, and LoVo-1 cells (IC50s = 1.67, 5.48, 7.16, and 20.03 μM, respectively) as well as human umbilical vein endothelial cells (HUVECs) but not human embryonic lung fibroblast cells (HELs; IC50s = 16.06 and >50 μM, respectively). Questiomycin A reduces the increased intracellular pH in a variety of cancer cell lines, as well as in HUVECs and HELs. It prevents lung metastasis in a B16 mouse melanoma model of metastasis when administered at a dose of 0.5 mg/kg simultaneously with B16 cells or every three days. It is also a chromophore product of the reducing agent 2-aminophenol oxidation (as 2-amino-phenoxazine-3-one) and has been used as a readout in the study of catalytic oxidation of 2-aminophenol by various metal-containing complexes. It has an absorbance of 435 nm in methanol.

Uses

Questiomycin A is a phenoxazine produced by several Streptomyces species and some fungi and bacteria. Questiomycin A is weakly active against bacteria, fungi, plants and tumour cell lines, and inhibits aromatase and sulfatases. Questiomycin, like other phenoxazines, stimulates cell growth and turnover in vitro, an activity possibly related to their ability to form stable free radicals. More recently, questiomycin A has been shown to inhibit pulmonary metastasis caused by mouse melanoma cells. Questiomycin A and related phenoxazines are important dereplication standards in discovery research to eliminate leads due to high amounts of weakly potent actives.

Uses

Questiomycin A is a natural antimicrobial agent produced by agriculturally important species such as maize, rye, and wheat.

Definition

ChEBI: 2-aminophenoxazin-3-one is a phenoxazine.

in vivo

Questiomycin A (10 mg/kg; i.p.; once a day for 8 days) enhances the antitumor effect of Sorafenib by stimulating the downregulation of GRP78 in vivo[1].

Animal Model:Nude mice (HepG2 cells xenograft model)[1].
Dosage:10 mg/kg
Administration:Intraperitoneal injection; once a day; 8 days.
Result:Stimulated Sorafenib-dependent antitumor activity.

References

[1] K ANZAI. The new antibiotics, questiomycins A and B.[J]. Journal of Antibiotics, 1960, 13: 125-132.
[2] NARAYAN CH. JANA . Synthesis, structure and diverse coordination chemistry of cobalt(III) complexes derived from a Schiff base ligand and their biomimetic catalytic oxidation of o-aminophenols[J]. Polyhedron, 2019, 164: Pages 23-34. DOI: 10.1016/j.poly.2019.02.024
[3] SHIGETAKA SHIMIZU. Phenoxazine compounds produced by the reactions with bovine hemoglobin show antimicrobial activity against non-tuberculosis mycobacteria.[J]. Tohoku Journal of Experimental Medicine, 2004, 203 1: 47-52. DOI: 10.1620/tjem.203.47
[4] XIAO-FANG CHE. 2-Aminophenoxazine-3-one and 2-amino-4,4α-dihydro-4α,7-dimethyl-3H-phenoxazine-3-one cause cellular apoptosis by reducing higher intracellular pH in cancer cells.[J]. Proceedings of the Japan Academy. Series B, Physical and Biological Sciences, 2011, 87 4: 199-213. DOI: 10.2183/pjab.87.199
[5] TAKAYUKI HONGO . 2-Aminophenoxazine-3-one Prevents Pulmonary Metastasis of Mouse B16 Melanoma Cells in Mice[J]. Journal of pharmacological sciences, 2010, 114 1: Pages 63-68. DOI: 10.1254/jphs.10023fp
[6] CHANDAN MUKHERJEE. A Tetracopper(II)-Tetraradical Cuboidal Core and Its Reactivity as a Functional Model of Phenoxazinone Synthase?[J]. Inorganic Chemistry, 2007, 46 23: 9895-9905. DOI: 10.1021/ic7012599

3-aminophenoxazone Preparation Products And Raw materials

Raw materials

Preparation Products

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3-aminophenoxazone Suppliers

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1916-59-2, 3-aminophenoxazoneRelated Search:


  • 3-aminophenoxazone
  • 2-Amino-3-oxo-3H-phenoxazine
  • 2-Aminophenoxazon
  • Phx-3
  • Questiomycin A
  • 2-AMino-3-phenoxazone
  • 3H-Phenoxazin-3-one, 2-aMino-
  • 2-AMino-3H-phenoxazin-3-one, 2-AMinophenoxazone, AV Toxin C, NSC 94945
  • 2-amino-1H-phenoxazin-3-one
  • 1916-59-2