3-METHYL-2-((E)-3-[1-[3-(TRIMETHYLAMMONIO)PROPYL]-4(1H)-PYRIDINYLIDENE]-1-PROPENYL)-1,3-BENZOTHIAZOL-3-IUM DIIODIDE

3-METHYL-2-((E)-3-[1-[3-(TRIMETHYLAMMONIO)PROPYL]-4(1H)-PYRIDINYLIDENE]-1-PROPENYL)-1,3-BENZOTHIAZOL-3-IUM DIIODIDE Property

solubility 

Soluble in dimethyl sulfoxide

form 

Solid

Appearance

purple solution

ex/em

575/599 nm

ε(extinction coefficient)

80900 cm−1M−1

Φ(quantum yield)

0.62

λmax

575 nm (H2O/DNA)

Major Application

Photonic fabric display

Biological Applications

Detecting nucleic acids, cells, microorganisms; nucleic acids amplification; as temperature sensor

Hazard and Precautionary Statements (GHS)

3-METHYL-2-((E)-3-[1-[3-(TRIMETHYLAMMONIO)PROPYL]-4(1H)-PYRIDINYLIDENE]-1-PROPENYL)-1,3-BENZOTHIAZOL-3-IUM DIIODIDE Chemical Properties,Usage,Production

Description

BO-TAP-3 (also known as BO-PRO^®-3) is a orange fluorescent carbocyanine monomeric dye. BO-TAP-3 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a multiple fluorescence enhancement upon binding to dsDNA.

The bright fluorescence signal and low background make BO-TAP-3 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments.

The dye is used to image cell nuclei and cytoplasm, label bacteria and sperms, perform nucleic acid detection and amplification, and as a temperature sensor.

Chemical Properties

ex/em: 575/599 nm
Extinction coefficient (ε): 80900 cm??1M??1
Quantum yield (Φ): 0.62

Abs/Em Maxima

564 (complex)/600 (complex) nm