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ACRIDINE ORANGE

Product Name
ACRIDINE ORANGE
CAS No.
65-61-2
Chemical Name
ACRIDINE ORANGE
Synonyms
AO;AOX1;Lysotraker;CI NO 46005;BASIC ORANGE;-Cellstain-Ao;Euchrysine 3r;EUCHRYSINE 3RX;acridineoranger;N’-Tetramethyl-3
CBNumber
CB0747468
Molecular Formula
C17H20ClN3
Formula Weight
301.82
MOL File
65-61-2.mol
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ACRIDINE ORANGE Property

Melting point:
284-287 °C(lit.)
Density 
1.001 g/mL at 20 °C
refractive index 
n20/D 1.338
storage temp. 
-20°C
solubility 
Soluble in water, ethanol, dimethyl sulfoxide
form 
Powder
pka
-3.2, 10.5(at 25℃)
Colour Index 
46005
color 
orange to red
λmax
500 nm
BRN 
3734978
Major Application
Adhesives; aluminophosphate crystalline materials; detecting clay particles; display device; evaluating fiber surface characteristics; glass matrixes; imaging material; inks; lasers; recording materials; photoresists; textiles; thin films; tracers for hydrology; wiring boards
Biological Applications
Detecting cancer cells,spores,human papilloma virus (HPV),stress biomarkers; treating amyloid associated diseases,lupus,pathogen infections; apoptosis assays; cytotoxicity assays
CAS DataBase Reference
65-61-2
EPA Substance Registry System
C.I. Basic Orange 14 (65-61-2)
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Safety

Safety Statements 
22-24/25
WGK Germany 
3
RTECS 
AR7601000
HS Code 
32041300
Toxicity
dnd-mus:ast 20 mmol/L BBACAQ 374,96,74
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H341Suspected of causing genetic defects

Precautionary statements

P201Obtain special instructions before use.

P202Do not handle until all safety precautions have been read and understood.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P308+P313IF exposed or concerned: Get medical advice/attention.

P405Store locked up.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
318337
Product name
Acridine Orange hydrochloride hydrate
Purity
99% (HPLC)
Packaging
1g
Price
$146
Updated
2024/03/01
Sigma-Aldrich
Product number
318337
Product name
Acridine Orange hydrochloride hydrate
Purity
99% (HPLC)
Packaging
5g
Price
$714
Updated
2024/03/01
Sigma-Aldrich
Product number
A9231
Product name
Acridine Orange solution
Packaging
10ml
Price
$165
Updated
2024/03/01
Sigma-Aldrich
Product number
318337
Product name
Acridine Orange hydrochloride hydrate
Purity
99% (HPLC)
Packaging
250mg
Price
$41.5
Updated
2024/03/01
Sigma-Aldrich
Product number
A8097
Product name
Acridine Orange hydrochloride solution
Purity
10mg/mL in H
Packaging
10ml
Price
$106
Updated
2024/03/01
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ACRIDINE ORANGE Chemical Properties,Usage,Production

Chemical Properties

dark orange to brown powder

Uses

Acridine orange is a dye that interacts with DNA and RNA by intercelation or electrostatic attraction. This cationic dye has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels.

Preparation

4,4 ‘-Bis (N, N-dimethyl) aminodiphenylmethane obtained dinitration of 4,4′ – bis (N, N-dimethyl) amino-2 ‘- dinitro-diphenyl methane reduction, and with an acid of heat, so that the diamino compound is cyclized product is oxidized and then cyclized and converted into zinc chloride double salt.

Biological Activity

acridine orange hydrochloride is a cell and organelle membrane permeable fluorescent dye that targets nucleic acids. acridine orange can either be incorporated into double helical nucleic acids (detected by green fluorescence at 530 nm), or bind electrostatically to phosphate groups of single-stranded molecules (detected by red fluorescence at 640 nm), which renders it the ability to differently stain dna and rna or single-stranded dna in situ. for unfixed cells, acridine orange staining can still be used as a simple, fast approach of obtaining information on cell cycle status and cell ploidy levels from dna staining (green fluorescence), as well as cell transcriptional activity from rna measurements (red fluorescence), in a flow cytofluorometric system. thus, acridine orange may serve as a useful cytochemical stain for cell-cycle and apoptosis studies.1. mcmaster gk, carmichael gg. analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. proceedings of the national academy of sciences of the united states of america, 1977, 74(11): 4835-4838.2. traganos f, darzynkiewicz z, sharpless t, et al. simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. journal of histochemistry and cytochemistry, 1977, 25(1): 46-56.

Safety Profile

Mutation data reported. Whenheated to decomposition it emits very toxic fumes of HCl,Cl??, and NOx.

Enzyme inhibitor

This cell-permeable dye (FWfree-base = 265.36 g/mol; CAS 65-61-2), also known as N,N,N',N'-tetramethylacridine-3,6-diamine, is an intercalator that binds tightly to nucleic acids. Binding to DNA is characterized by excitation maximum at 502 nm (cyan) and an emission maximum at 525 nm, producing a green fluorescence. (A 120 μM solution of acridine orange will detect 25-50 ng DNA per band in an agarose or polyacrylamide gel.) Binding to RNA is characterized by an excitation maximum at 460 nm and the emission maximum is located at 650 nm, yielding a reddish-orange fluorescence. Acridine orange also penetrates acidic compartments (e.g., lysosomes and phagolysosomes); upon protonation, the dye emits orange light when excited by blue light.

storage

Room temperature

Properties and Applications

bright yellow orange. Soluble in water and ethanol for orange yellow, with green fluorescent. The strong sulfuric acid in nearly colorless, a green fluorescent, diluted for orange. The dye solution with strong sodium hydroxide solution have yellow precipitation. And C.I. Solvent Orange 15 the same chemical structure (free base).

Standard( Silk ) Light Fastness Persperation Fastness Ironing Fastness Soaping
Fading Stain Fading Stain Fading Stain
ISO 1-2 3 1 5 1 3

ACRIDINE ORANGE Preparation Products And Raw materials

Raw materials

Preparation Products

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ACRIDINE ORANGE Suppliers

Americas 1 Belgium 1 China 89 Europe 3 France 1 Germany 1 India 7 Japan 4 Switzerland 2 United Kingdom 4 United States 22 Global 135
MedChemexpress LLC
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View Lastest Price from ACRIDINE ORANGE manufacturers

Henan Fengda Chemical Co., Ltd
Product
ACRIDINE ORANGE 65-61-2
Price
US $5.00-0.20/KG
Min. Order
1KG
Purity
98%
Supply Ability
g-kg-tons, free sample is available
Release date
2024-03-26

65-61-2, ACRIDINE ORANGERelated Search:

ACRIFLAVINE ACRIDINE HYDROCHLORIDE PROFLAVINE HEMISULFATE ACRIDINE ORANGE 3,6-DIAMINOACRIDINE HYDROCHLORIDE 7-Aminoquinoline Basic Orange 14 Acridine ACRIDINE ORANGE (C.I. 46005:1),ACRIDINE ORANGE BASIC,ACRIDINE ORANGE BASE ACRIDINE ORANGE 10-NONYL BROMIDE,NONYL ACRIDINE ORANGE ACRIDINE ORANGE-10-OCTADECYL BROMIDE,3,6-Bis(dimethylamino)-10-octadecylacridinium bromide, Acridine orange-10-octadecyl bromide ACRIDINE ORANGE-10-DODECYL BROMIDE,3,6-Bis(dimethylamino)-10-dodecylacridinium bromide, Acridine orange-10-dodecyl bromide ACRIDINE ORANGE, 1/2ZNCL DIMETHYL-QUINOLIN-7-YL-AMINE ACRIDINE ORANGE HEMI (ZINC CHLORIDE) ELECTROPHORESIS GRADE ACRIDINE ORANGE, ELECTRO PURE, CI 46005 Acridine Orange in Acetate Buffer ACRIDINE ORANGE 10-NONYL BROMIDE (NONYL ACRIDINE ORANGE)

  • 3,6-BIS(DIMETHYLAMINO)ACRIDINE HYDROCHLORIDE
  • ACRIDINE ORANGE (E GURR)
  • ACRIDINE ORANGE HYDROCHLORIDE
  • ACRIDINE ORANGE N
  • BASIC ORANGE
  • BIS(DIMETHYLAMINO) ACRIDINE HYDROCHLORIDE
  • AO
  • CI NO 46005
  • EUCHRYSINE 3RX
  • rhodulineorangeno
  • N,N,N',N'-tetramethylacridin-3,6-yldiamine hydrochloride
  • ACRIDINE ORANGE SOLUTION
  • ACRIDINE ORANGE HYDROCHLORIDE SOLUTION, ~1% IN WATER
  • ACRIDINE ORANGE FOR MICROSCOPY
  • ACRIDINE ORANGE HYDROCHLORIDE HYDRATE, F OR MICROSCOPY
  • ACRIDINE ORANGE HYDROCHLORIDE HYDRATE 80%
  • -Cellstain-Ao
  • -Cellstain-AoSolution
  • ACRIDING ORANGE HYDROCHLORIDE
  • 3,6-Bis(dimethylamino) acridine, zinc chloride double salt
  • Acridine Orange, hydrochloride hydrate, ca. 80 % dye content, pure
  • AOX1
  • acridine orange hydrochloride solution
  • 3,6-Bis(dimethylamino)acridine,hydrochloride,hydrate,aqueoussolution
  • ACRIDINE ORANGE N-HYRATE
  • Basic orange 14 (C.I. 46005)
  • Acridine Orange solution hydrochloride
  • Acridine Orange hydrate hydrochloride
  • Acridine Orange, hydrochloride hydrate,pure,ca. 80 % dye content
  • Acridine Orange hydrochloride solution,3,6-Bis(dimethylamino)acridine hydrochloride
  • ACRIDINE ORANGE HYDROCHLORIDE HYDRATE PURUM, FOR MICROSCOPY ((FL., HIST.)) 98%
  • 6-acridinediamine
  • 6-Bis(dimethylamino)acridine hydrochloride 3
  • Lysotraker
  • N’-Tetramethyl-3
  • N,N,N',N'-Tetramethyl-3,6-acridinediamine hydrochloride
  • RHODULINE ORANGE
  • 3,6-BIS(DIMETHYLAMINO)ACRIDINE, HCL
  • 3,6-acridinediamine, N,n,n0,n0-tetramethyl-, monohydrochloride
  • Acridine, 3,6-bis(dimethylamino)-, Hydrochloride
  • Acridine, 3,6-bis(dimethylamino)-, Monohydrochloride
  • Euchrysine 3r
  • Sumitomo Acridine Orange No
  • Sumitomo acridine Orange Rk Conc
  • N3,N3,N6,N6-Tetramethylacridine-3,6-diamine hydrochloride
  • Acridine Orange hydrochloride?, >99%
  • N3,N3,N6,N6-tetramethylacridine-3,6-diamine chloride
  • 3,6-Acridinediamine,N,N,N’,N’-tetramethyl-,monohydrochloride
  • 3,6-bis(dimethylamino)-acridinmonohydrochloride
  • 6-acridinediamine,n,n,n’,n’-tetramethyl-monohydrochloride
  • acridineorangeno
  • acridineorangens
  • acridineoranger
  • c.i.basicorange14
  • Cellstain AO Solution (1mg/ml H2O)
  • Acridine Orange Solution [for Cell Staining]
  • C.I.Basic Orange 14:C.I.46005
  • Acridine orange hydrochloride, hydrate, ≥98% (HPLC)