ChemicalBook > CAS DataBase List > GELATINASE A

GELATINASE A

Product Name
GELATINASE A
CAS No.
193807-58-8
Chemical Name
GELATINASE A
Synonyms
MMPL;MMP-2;MMP-1;EC 3.4.24.7;EC 3.4.24.3;MMP-2, HUMAN;EC 3.4.24.24;EC: 3.4.24.3;GELATINASE A;COLLAGENASE D
CBNumber
CB1309850
Molecular Formula
C21H19NO4S
Formula Weight
381.44
MOL File
193807-58-8.mol
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GELATINASE A Property

Melting point:
146-147 °C
Boiling point:
595.9±60.0 °C(Predicted)
Density 
1.297±0.06 g/cm3(Predicted)
storage temp. 
2-8°C
solubility 
≤25mM in DMSO
pka
3.26±0.10(Predicted)
form 
powder
color 
light brown
CAS DataBase Reference
193807-58-8
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Safety

Hazard Codes 
B,Xi
Risk Statements 
36/37/38-42
Safety Statements 
23-26-36-24/25
WGK Germany 
2
10-21
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
SRP3118
Product name
MMP-2 human
Purity
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Packaging
10μG
Price
$353
Updated
2024/03/01
Sigma-Aldrich
Product number
M9445
Product name
Matrix Metalloproteinase-2 from mouse
Purity
recombinant, expressed in NSO cells, >95% (SDS-PAGE), buffered aqueous glycerol solution
Packaging
10μg
Price
$791
Updated
2024/03/01
Sigma-Aldrich
Product number
M9195
Product name
Matrix Metalloproteinase-1 human
Purity
recombinant, expressed in NSO cells, >95% (SDS-PAGE), buffered aqueous solution
Packaging
10μg
Price
$792
Updated
2024/03/01
Sigma-Aldrich
Product number
444213
Product name
MMP-2, Proenzyme, Human Rheumatoid Synovial Fibroblast
Packaging
5μg
Price
$257
Updated
2024/03/01
Sigma-Aldrich
Product number
444208
Product name
MMP-1, Proenzyme, Human Rheumatoid Synovial Fibroblast
Packaging
5μg
Price
$329
Updated
2024/03/01
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GELATINASE A Chemical Properties,Usage,Production

Uses

Matrix metalloproteinase-2 (MMP2) human has been used:

  • As a standard in zymography to measure gelatinolytic activity of MMP2.
  • In enzymatic method for dissociation of brain tissue to single cells.

General Description

Matrix metalloproteinase-2 (MMP-2) also known as gelatinase or type IV collagenase is a 72kDa protein. MMP-2 is a member of matrix metalloproteinase (MMP) family of enzymes. Basic structure of MMP2 contains signal peptide domain that targets the enzyme for secretion, the pro-peptide domain, which is removed when the enzyme is activated and the catalytic site containing gelatin-binding domain.

Biological Activity

mmp-2/mmp-9 inhibitor i is a potent inhibitor of matrix metalloproteinase-2 (mmp-2) and mmp-9.matrix metalloproteinase (mmp), a typical metalloproteinase, requires zinc ion at its active sites. as many as 18 kinds of mmp have been identified and cloned and are collectively called the mmp family.

Biochem/physiol Actions

Matrix Metalloproteinase-2 (MMP-2) cleaves gelatin, type IV, V, VII, X, and XI collagens, fibronectin, elastin, laminin, proteoglycans and a range of non extracellular matrix (ECM ) components. MMP-2 cleaves native type I collagen to N-terminal ? and C-terminal ? fragments identical to those generated by interstitial collagenases. MMP2 and MMP9 play an essential role in matrix degradation and they are implicated in the maintenance of neovascularization. In mice, deletion or inhibition of MMP2 protects against myocardial rupture.

in vitro

mmp-2/mmp-9 inhibitor i was identified as a potent inhibitor of matrix metalloproteinase-2 (mmp-2) and mmp-9 with ic50 values of 310 and 240 nm, respectively. mmp-2/mmp-9 inhibitor i acted by binding zinc at the active site of these mmps. mmp-2/mmp-9 inhibitor i was found to be able to block mmp-2/mmp-9-dependent invasion in cell culture model [1].

in vivo

both hydroxamic acid and carboxylic acid analogs of mmp-2/mmp-9 inhibitor i were evaluated for their inhibitory activities in animal cancer models. results showed that lung colonization of lewis lung carcinoma cells was suppressed by these inhibitors significantly. in addition, antitumor activity was also observed in the human lung cancer model. ma44 cells growed as a solid tumor on the peritoneum after being implanted ip, and mice bearing ma44 eventually died within 3 to 4 weeks. daily oral administration of compound 5l led to prolonged survival of ma44-bearing mice [1].

IC 50

310 and 240 nm for mmp-2 and mmp-9, respectively

References

[1] tamura, y. ,watamane, f.,nakatani, t., et al. highly selective and orally active inhibitors of type iv collagenase (mmp-9 and mmp-2): n-sulfonylamino acid derivatives. j. med. chem. 41(4), 640-649 (1998).

GELATINASE A Preparation Products And Raw materials

Raw materials

Preparation Products

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GELATINASE A Suppliers

Wuhan Shanhai Zhihe Biotechnology Co., Ltd.
Tel
17771424646
Email
shoubull@126.com
Country
China
ProdList
969
Advantage
58
Alabiochem Tech.Co., Ltd.
Tel
0512-58900862 400-0707518
Fax
0086-512-56765862
Email
sales@alabiochem.com
Country
China
ProdList
995
Advantage
59
Chengdu RunZeBenTu Chemical Co., Ltd
Tel
13096311329 028-88469284 616445927
Fax
028-88469284
Email
616445927@qq.com
Country
China
ProdList
2874
Advantage
50
Sigma-Aldrich
Tel
021-61415566 800-8193336
Email
orderCN@merckgroup.com
Country
China
ProdList
51456
Advantage
80
Shandong Xiya Chemical Co., Ltd.
Tel
4009903999 13395398332
Fax
0539-6365991
Email
sales@xiyashiji.com
Country
China
ProdList
20806
Advantage
60
Alabiotech Inc.
Tel
001-619-354-5251
Fax
001-855-855-6087
Email
sales@alabiotech-e.com
Country
United States
ProdList
972
Advantage
60
Shanghai Chaolan Chemical Technology Center
Tel
QQ:65489617 15618227136
Fax
21-5161 9052
Email
Sales@ATKchemical.com
Country
China
ProdList
7266
Advantage
58
Alpha Biopharmaceuticals Co., Ltd
Tel
+86-15542445688
Fax
0086-411-39042693
Email
sales@alabiochem.com
Country
China
ProdList
888
Advantage
58
Alpha Biopharmaceuticals Co., Ltd
Tel
0411-39042497
Fax
0411-39042693
Email
sales@alphabiopharm.com
Country
China
ProdList
1951
Advantage
58
Hefei Bomei Biotechnology Co., Ltd.
Tel
13856598764
Email
531626407@qq.com
Country
China
ProdList
3007
Advantage
58

193807-58-8, GELATINASE ARelated Search:


  • Collagenase Type IV 72 kDa
  • Gelatinase, 72 kDa
  • Collagenase Type IV 72 kD
  • 72 Kd Gelatinase 72 Kd Type IV collagenase Gelatinase A
  • Matrix Metalloproteinase-2 human
  • PROENZYME MMP-2
  • PROGELATINASE A
  • MMP-2
  • MMP-1 PROENZYME
  • MMP-1, PROENZYME HUMAN RHEUMATOID SYNOVIAL FIBROBLAST
  • MMP-1, STABLE HUMAN FIBROBLASTS
  • MMP-1 ENZYME, HUMAN
  • MMP-1, HUMAN PROTEIN
  • MMP-1
  • MMPL
  • MMP-2, PROENZYME
  • MMP-2, PROENZYME HUMAN RHEUMATOID SYNOVIAL FIBROBLAST
  • MMP-2, PROENZYME MOUSE MACROPHAGE
  • MMP-2 ENZYME, HUMAN
  • MMP-2, HUMAN
  • TYPE IV COLLAGENASE
  • TYPE 1 COLLAGENASE
  • 72 KDA GELATINASE
  • 72 KDA GELATINASE, PROENZYME
  • 72 KDA TYPE IV COLLAGENASE
  • 72 KDA TYPE IV COLLAGENASE, PROENZYME
  • 72 KD GELATINASE
  • 72 KD TYPE IV COLLAGENASE
  • ACTIVE MMP-2 ENZYME
  • MATRIX METALLOPROTEINASE-1
  • MATRIX METALLOPROTEINASE 1, PROENZYME
  • MATRIX METALLOPROTEINASE-1, STABLE HUMAN FIBROBLASTS
  • MATRIX METALLOPROTEINASE 2
  • MATRIX METALLOPROTEINASE-2 (MOUSE FIBROBLASTS)
  • MATRIX METALLOPROTEINASE 2, PROENZYME
  • MATRIX METALLOPROTEINASE, PROENZYME
  • EC 3.4.24.7
  • EC 3.4.24.24
  • EC 3.4.24.3
  • EC: 3.4.24.3
  • COLLAGENASE D
  • COLLAGENASE-1
  • COLLAGENASE 1 PROENZYME
  • COLLAGENASE-1, STABLE HUMAN FIBROBLASTS
  • COLLAGENASE FORM II
  • COLLAGENASE (GRADE A)
  • 72 kD Gelatinase, 72 kD Type IV Collagenase, Gelatinase A, MMP-2
  • Matrix Metalloproteinase-2 from mouse fibroblasts
  • Collagenase-1, Interstitial Collagenase, MMP-1
  • Matrix Metalloproteinase-1, Stable from human fibroblasts
  • Matrix Metalloproteinase-2 from human fibroblasts
  • Matrix Metalloproteinase-2 from mouse
  • Matrix Metalloproteinase-1 human
  • Matrix Metalloproteinase-2 from rat fibroblasts
  • COLLAGENASE (GRADE B)
  • COLLAGENASE H
  • COLLAGENASE (72KDA TYPE IV) PROENZYME
  • COLLAGENASE AND PROTEASE INHIBITOR