GELATINASE A

Product Name: GELATINASE A
CAS No.: 193807-58-8
Chemical Name: GELATINASE A
Synonyms: MMPL;MMP-2;MMP-1;EC 3.4.24.7;EC 3.4.24.3;MMP-2, HUMAN;EC 3.4.24.24;EC: 3.4.24.3;GELATINASE A;COLLAGENASE D
CBNumber: CB1309850
Molecular Formula: C21H19NO4S
Formula Weight: 381.44
MOL File: 193807-58-8.mol

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GELATINASE A Property

Melting point:: 146-147 °C
Boiling point:: 595.9±60.0 °C(Predicted)
Density: 1.297±0.06 g/cm3(Predicted)
storage temp.: 2-8°C
solubility: ≤25mM in DMSO
pka: 3.26±0.10(Predicted)
form: powder
color: light brown
biological source: human
Water Solubility: water: soluble
Specific Activity: ≥600mU/mg protein
CAS DataBase Reference: 193807-58-8

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Safety

Hazard Codes: B,Xi
Risk Statements: 36/37/38-42
Safety Statements: 23-26-36-24/25
WGK Germany: 2
F: 10-21

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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: SRP3118
Product name: MMP-2 human
Purity: recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Packaging: 10μG
Price: $353
Updated: 2024/03/01

Sigma-Aldrich

Product number: M9445
Product name: Matrix Metalloproteinase-2 from mouse
Purity: recombinant, expressed in NSO cells, >95% (SDS-PAGE), buffered aqueous glycerol solution
Packaging: 10μg
Price: $791
Updated: 2024/03/01

Sigma-Aldrich

Product number: M9195
Product name: Matrix Metalloproteinase-1 human
Purity: recombinant, expressed in NSO cells, >95% (SDS-PAGE), buffered aqueous solution
Packaging: 10μg
Price: $792
Updated: 2024/03/01

Sigma-Aldrich

Product number: 444213
Product name: MMP-2, Proenzyme, Human Rheumatoid Synovial Fibroblast
Packaging: 5μg
Price: $257
Updated: 2024/03/01

Sigma-Aldrich

Product number: 444208
Product name: MMP-1, Proenzyme, Human Rheumatoid Synovial Fibroblast
Packaging: 5μg
Price: $329
Updated: 2024/03/01

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GELATINASE A Chemical Properties,Usage,Production

Uses

Matrix metalloproteinase-2 (MMP2) human has been used:

As a standard in zymography to measure gelatinolytic activity of MMP2.
In enzymatic method for dissociation of brain tissue to single cells.

General Description

Matrix metalloproteinase-2 (MMP-2) also known as gelatinase or type IV collagenase is a 72kDa protein. MMP-2 is a member of matrix metalloproteinase (MMP) family of enzymes. Basic structure of MMP2 contains signal peptide domain that targets the enzyme for secretion, the pro-peptide domain, which is removed when the enzyme is activated and the catalytic site containing gelatin-binding domain.

Biological Activity

mmp-2/mmp-9 inhibitor i is a potent inhibitor of matrix metalloproteinase-2 (mmp-2) and mmp-9.matrix metalloproteinase (mmp), a typical metalloproteinase, requires zinc ion at its active sites. as many as 18 kinds of mmp have been identified and cloned and are collectively called the mmp family.

Biochem/physiol Actions

Matrix Metalloproteinase-2 (MMP-2) cleaves gelatin, type IV, V, VII, X, and XI collagens, fibronectin, elastin, laminin, proteoglycans and a range of non extracellular matrix (ECM ) components. MMP-2 cleaves native type I collagen to N-terminal ? and C-terminal ? fragments identical to those generated by interstitial collagenases. MMP2 and MMP9 play an essential role in matrix degradation and they are implicated in the maintenance of neovascularization. In mice, deletion or inhibition of MMP2 protects against myocardial rupture.

in vitro

mmp-2/mmp-9 inhibitor i was identified as a potent inhibitor of matrix metalloproteinase-2 (mmp-2) and mmp-9 with ic50 values of 310 and 240 nm, respectively. mmp-2/mmp-9 inhibitor i acted by binding zinc at the active site of these mmps. mmp-2/mmp-9 inhibitor i was found to be able to block mmp-2/mmp-9-dependent invasion in cell culture model [1].

in vivo

both hydroxamic acid and carboxylic acid analogs of mmp-2/mmp-9 inhibitor i were evaluated for their inhibitory activities in animal cancer models. results showed that lung colonization of lewis lung carcinoma cells was suppressed by these inhibitors significantly. in addition, antitumor activity was also observed in the human lung cancer model. ma44 cells growed as a solid tumor on the peritoneum after being implanted ip, and mice bearing ma44 eventually died within 3 to 4 weeks. daily oral administration of compound 5l led to prolonged survival of ma44-bearing mice [1].

IC 50

310 and 240 nm for mmp-2 and mmp-9, respectively

References

[1] tamura, y. ,watamane, f.,nakatani, t., et al. highly selective and orally active inhibitors of type iv collagenase (mmp-9 and mmp-2): n-sulfonylamino acid derivatives. j. med. chem. 41(4), 640-649 (1998).

GELATINASE A Preparation Products And Raw materials

Raw materials

Preparation Products

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GELATINASE A Suppliers

China 61 Europe 1 Germany 3 Japan 3 Switzerland 2 United Kingdom 1 United States 20 Global 91

Alabiotech Inc.

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MP Biomedicals, Inc.

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Abcam

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Cayman Chemical Company

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Creative Enzymes

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Roche Applied Science

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Kamiya Biomedical Company

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BIOMOL INTERNATIONAL, LP

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Worthington Biochemical Corporation

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EMD Biosciences, Inc.

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193807-58-8, GELATINASE ARelated Search:

COLLAGENASE TYPE II Peroxidase(POD) TRYPSIN FROM BOVINE PANCREAS Snailase　 PEPSIN LYSOZYME RNASEA HYALURONIDASE Collagenase

Collagenase Type IV 72 kDa

Gelatinase, 72 kDa

Collagenase Type IV 72 kD

72 Kd Gelatinase 72 Kd Type IV collagenase Gelatinase A

Matrix Metalloproteinase-2 human

PROENZYME MMP-2

PROGELATINASE A

MMP-2

MMP-1 PROENZYME

MMP-1, PROENZYME HUMAN RHEUMATOID SYNOVIAL FIBROBLAST

MMP-1, STABLE HUMAN FIBROBLASTS

MMP-1 ENZYME, HUMAN

MMP-1, HUMAN PROTEIN

MMP-1

MMPL

MMP-2, PROENZYME

MMP-2, PROENZYME HUMAN RHEUMATOID SYNOVIAL FIBROBLAST

MMP-2, PROENZYME MOUSE MACROPHAGE

MMP-2 ENZYME, HUMAN

MMP-2, HUMAN

TYPE IV COLLAGENASE

TYPE 1 COLLAGENASE

72 KDA GELATINASE

72 KDA GELATINASE, PROENZYME

72 KDA TYPE IV COLLAGENASE

72 KDA TYPE IV COLLAGENASE, PROENZYME

72 KD GELATINASE

72 KD TYPE IV COLLAGENASE

ACTIVE MMP-2 ENZYME

MATRIX METALLOPROTEINASE-1

MATRIX METALLOPROTEINASE 1, PROENZYME

MATRIX METALLOPROTEINASE-1, STABLE HUMAN FIBROBLASTS

MATRIX METALLOPROTEINASE 2

MATRIX METALLOPROTEINASE-2 (MOUSE FIBROBLASTS)

MATRIX METALLOPROTEINASE 2, PROENZYME

MATRIX METALLOPROTEINASE, PROENZYME

EC 3.4.24.7

EC 3.4.24.24

EC 3.4.24.3

EC: 3.4.24.3

COLLAGENASE D

COLLAGENASE-1

COLLAGENASE 1 PROENZYME

COLLAGENASE-1, STABLE HUMAN FIBROBLASTS

COLLAGENASE FORM II

COLLAGENASE (GRADE A)

72 kD Gelatinase, 72 kD Type IV Collagenase, Gelatinase A, MMP-2

Matrix Metalloproteinase-2 from mouse fibroblasts

Collagenase-1, Interstitial Collagenase, MMP-1

Matrix Metalloproteinase-1, Stable from human fibroblasts

Matrix Metalloproteinase-2 from human fibroblasts

Matrix Metalloproteinase-2 from mouse

Matrix Metalloproteinase-1 human

Matrix Metalloproteinase-2 from rat fibroblasts

COLLAGENASE (GRADE B)

COLLAGENASE H

COLLAGENASE (72KDA TYPE IV) PROENZYME

COLLAGENASE AND PROTEASE INHIBITOR