FLUORESCEIN-12-DUTP

Product Name: FLUORESCEIN-12-DUTP
Chemical Name: FLUORESCEIN-12-DUTP
Synonyms: FLUOR-12-DUTP;FLUORESCEIN-12-DUTP;CHROMATIDE(TM) FLUORESCEIN-12-DUTP;FLUORESCEIN-6-CARBOXAMINOCAPROYL-[5-[3-AMINOALLYL]-2'-DEOXYURIDINE-5'-TRIPHOSPHATE]
CBNumber: CB1341058
Molecular Formula: C38H41N4O19P3
Formula Weight: 950.67
MOL File: Mol file

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FLUORESCEIN-12-DUTP Property

storage temp.: -20°C
solubility: Water: soluble
form: solution

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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 11373242910
Product name: Fluorescein-12-dUTP
Purity: ≥85% (HPLC), suitable for ELISA, suitable for hybridization, solution
Packaging: 25μL
Price: $345
Updated: 2025/07/31

American Custom Chemicals Corporation

Product number: FRL0000572
Product name: FLUORESCEIN-12-DUTP
Purity: 95.00%
Packaging: 5MG
Price: $497.9
Updated: 2021/12/16

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FLUORESCEIN-12-DUTP Chemical Properties,Usage,Production

Uses

Fluorescein-12-dUTP is used as a substrate for:

terminal transferase
DNA polymerase I (holoenzyme and Klenow fragment),
Taq DNA polymerase
reverse transcriptase (e.g., from AMV and M-MuLV).

Fluorescein-12-dUTP has been used to label nuclear extracts during PCR. It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL. Fluorescein-labeled probes can be used for in in situ hybridization with direct fluorescence detection or detection by enzyme linked immunoimmunsorbent assay (ELISA) using Anti-Fluorescein-AP, Fab fragments. Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.

References

[1] J KOCH. Fast one-step procedure for the detection of nucleic acids in situ by primer-induced sequence-specific labeling with fluorescein-12-dUTP.[J]. Cytogenetics and cell genetics, 1992, 60 1: 1-3. DOI: 10.1159/000133281

FLUORESCEIN-12-DUTP Preparation Products And Raw materials

Raw materials

Preparation Products

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FLUORESCEIN-12-DUTP Suppliers

CHINA 29 United States 6 Global 35

Sigma-Aldrich

Tel: 021-61415566 800-8193336
Email: orderCN@merckgroup.com
Country: China
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BOC Sciences

Tel: 16314854226
Fax: 1-631-614-7828
Email: inquiry@bocsci.com
Country: United States
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BOC Sciences

Tel: +1-631-485-4226
Fax: 1-631-614-7828
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Country: United States
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Cayman Chemical Company

Tel: 800-364-9897
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Country: China
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Merck KGaA

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Country: China
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eENZYME LLC

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Shanghai Xintian Biotechnology Co., Ltd.

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Lingdu Industrial (Shanghai) Co., Ltd.

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Guangzhou Tuoshan Biotechnology Co., Ltd.

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Country: CHINA
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Nanjing Weijia Biotechnology Co., Ltd.

Tel: 13372024297
Country: CHINA
ProdList: 157
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FLUORESCEIN-12-DUTPRelated Search:

2'-Deoxyuridine

CHROMATIDE(TM) FLUORESCEIN-12-DUTP

FLUORESCEIN-12-DUTP

FLUOR-12-DUTP

FLUORESCEIN-6-CARBOXAMINOCAPROYL-[5-[3-AMINOALLYL]-2'-DEOXYURIDINE-5'-TRIPHOSPHATE]