Bromocresol Purple Property

Melting point:

240 °C (dec.) (lit.)

Density 

1.6509 (estimate)

Flash point:

36 °C

storage temp. 

Store at +5°C to +30°C.

solubility 

ethanol: soluble1mg/mL

form 

Solid

pka

6.21, 6.3, 6.4(at 25℃)

Boiling point:

590.2±50.0 °C(Predicted)

color 

Purplish-brown

PH Range

5.2(yellow)-6.8(purple)

PH

pH : 5.2～6.8

Water Solubility 

practically insoluble

λmax

419nm, 433nm, 591nm

Merck 

14,1387

BRN 

359618

Stability:

Stable. Incompatible with strong oxidizing agents.

Major Application

Waveguides, fuel cells, sensors, sol-gel technology, display device, falsification-proof security paper, paints, detergents, identifying fresh and stale rice, detecting lactic acid bacteria, dental materials, vaginal infection test method

InChIKey

ABIUHPWEYMSGSR-UHFFFAOYSA-N

CAS DataBase Reference

115-40-2(CAS DataBase Reference)

EPA Substance Registry System

Bromcresol Purple (115-40-2)

Safety

Hazard Codes 

Xi,Xn

Risk Statements 

36/37/38-10-20/21/22

Safety Statements 

26-36-37/39-16

RIDADR 

UN 1993 3/PG 3

WGK Germany 

3

TSCA 

Yes

HS Code 

29349990

Hazardous Substances Data

115-40-2(Hazardous Substances Data)

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P405Store locked up.

Bromocresol Purple Chemical Properties,Usage,Production

Chemical Properties

Crystalline

Uses

As indicator, pH 5.2 yellow; pH 6.8 purple.

Uses

It is used as a pH indicator and to measure serum albumin concentrations. It is used as a pH indicator (5.2-6.8 : yellow to blue-purple) and also used in spectrophotometric determination of serum-albumin.

Uses

Bromocresol purple has been used as a pH indicator in the yeast culture media. It has been used as a pH indicator in the culture media of Geobacillus stearothermophilus subsp. Calidolactis.

Definition

ChEBI: A member of the class of 2,1-benzoxathioles that is 2,1-benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 3-bromo-4-hydroxy-5-methylphenyl groups. A hydrophilic dye that is used as a pH indicator and to measure serum albumin concentrations.

General Description

Bromocresol purple is a pH indicator, which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It aids in determining the dead cell count for cells with plasma membrane damage. Bromocresol purple is widely used as a fluorescent stain in yeast cells. Cells with an intact plasma membrane are not stained, while damaged cells are seen as blue-grey ghosts.

Biochem/physiol Actions

Bromocresol purple is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.

Purification Methods

Dissolve the dye in aqueous 5% NaHCO3 solution and precipitate it from a hot solution by dropwise addition of aqueous HCl. Repeat this until the UV/VIS-extinction did not increase at 419nm. It can also be recrystallised from *benzene. It is an indicator: at pH 5.2 (yellow) and pH 6.8 (purple). [Beilstein 19/3 V 460.]