ChemicalBook > CAS DataBase List > PYRONIN Y

PYRONIN Y

Product Name
PYRONIN Y
CAS No.
92-32-0
Chemical Name
PYRONIN Y
Synonyms
Pyonin G;Pyonin Y;CI 45005;pyronine;PYRONIN G;PYRONIN J;PYRONIN Y;PYRONINE G;PYRONINE Y;PYRONIN GS
CBNumber
CB1418608
Molecular Formula
C17H19ClN2O
Formula Weight
302.8
MOL File
92-32-0.mol
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PYRONIN Y Property

Melting point:
250-260 °C(lit.)
storage temp. 
room temp
solubility 
Soluble in water; sparingly soluble in ethanol, ethylene glycol, methyl cellosolve
Colour Index 
45005
form 
Powder/Solid
color 
Pink
Water Solubility 
Soluble in water. Also soluble in methyl cellosolve (9 mg/mL) and ethanol (3 mg/mL).
λmax
548 nm
Merck 
13,8097
BRN 
3795789
Stability:
Stable. Incompatible with strong oxidizing agents.
Biological Applications
Antimalarial agent; antiviral agent; nucleic acid sequencing; treating protozoan infections; herbicides
InChIKey
INCIMLINXXICKS-UHFFFAOYSA-M
CAS DataBase Reference
92-32-0
EPA Substance Registry System
Pyronine Y (92-32-0)
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Safety

Hazard Codes 
Xn,Xi
Risk Statements 
68-36/37/38
Safety Statements 
22-24/25-36-26
WGK Germany 
3
RTECS 
BQ1450000
TSCA 
Yes
HS Code 
32041300
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Precautionary statements

P201Obtain special instructions before use.

P202Do not handle until all safety precautions have been read and understood.

P281Use personal protective equipment as required.

P308+P313IF exposed or concerned: Get medical advice/attention.

P403Store in a well-ventilated place.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
213519
Product name
Pyronin Y
Purity
certified by the Biological Stain Commission, Dye content 50%
Packaging
1g
Price
$52.3
Updated
2024/03/01
Sigma-Aldrich
Product number
213519
Product name
Pyronin Y
Purity
certified by the Biological Stain Commission, Dye content 50%
Packaging
5g
Price
$170
Updated
2024/03/01
Alfa Aesar
Product number
J60505
Product name
Pyronin Y, 0.2% w/v aq. soln.
Packaging
10ml
Price
$35.5
Updated
2021/12/16
Alfa Aesar
Product number
J60505
Product name
Pyronin Y, 0.2% w/v aq. soln.
Packaging
25ml
Price
$46.6
Updated
2021/12/16
Cayman Chemical
Product number
14488
Product name
Pyronin Y
Packaging
1g
Price
$32
Updated
2024/03/01
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PYRONIN Y Chemical Properties,Usage,Production

Chemical Properties

green crystalline solid

Uses

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

Uses

Pyronin Y has been used:

  • in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
  • as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
  • staining of CD34+ cells

Uses

Bacterial and biological stain.

Definition

ChEBI: An organic chloride salt having 6-(dimethylamino)-N,N-dimethyl-3H-xanthen-3-iminium as the cation. Used with methyl green to selectively demonstrate RNA (red) in contrast to DNA (green) with the Unna Pappenheim method.

General Description

Pyronin Y (C.I. 45005) is an aminoxanthene dye that is present as a weakly lipophilic cation in neutral aqueous solutions. It is also known as pyronine G.

Biochem/physiol Actions

Pyronin Y is important in histochemistry and is combined with methyl green for staining of RNA. The mixture results in bright red/green staining of nucleic acids.

storage

Room temperature

Purification Methods

Commercial material may contain a large quantity of zinc. Purify it by dissolving 1g in 50mL of hot water containing 5g NaEDTA. Cool to 0o, filter, evaporate to dryness and the residue is extracted with EtOH. The solution is evaporated to 5-10mL, filtered, and the dye is precipitated by addition of excess of dry diethyl ether. It is centrifuged, and the crystals are washed with dry ether. The procedure is repeated, then the product is dissolved in CHCl3, filtered and evaporated. The dye is stored in a vacuum. [Beilstein 18 H 596, 18 III/IV 7361, 18/10 V 181.]

PYRONIN Y Preparation Products And Raw materials

Raw materials

Preparation Products

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PYRONIN Y Suppliers

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View Lastest Price from PYRONIN Y manufacturers

Henan Fengda Chemical Co., Ltd
Product
PYRONIN Y 92-32-0
Price
US $35.00-1.40/kg
Min. Order
1kg
Purity
99%
Supply Ability
g-kg-tons, free sample is available
Release date
2024-04-02
Career Henan Chemical Co
Product
Pyronin Y 92-32-0
Price
US $1.00/kg
Min. Order
1kg
Purity
95%-99%
Supply Ability
1000kg
Release date
2018-12-25

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